Title
An antifibrin monoclonal antibody useful in immunoscintigraphic detection of thrombi
Author
Wasser, M.N.J.M.
Koppert, P.W.
Arndt, J.W.
Emeis, J.J.
Feitsma, R.I.J.
Pauwels, E.K.J.
Nieuwenhuizen, W.
Gaubius Instituut TNO
Publication year
1989
Abstract
Balb/c mice were immunized with human plasmin-generated fibrinogen degradation product Y. Spleen cells were fused with P3X63-Ag8.653 myeloma cells. A clone (Y22) was found that produces monoclonal antibodies (MoAbs) with a strong reactivity with human fibrin and only a weak reactivity with fibrinogen in an enzyme immunoassay (EIA). Y22 also reacts with fibrin of rabbits, rats, sheep, and dogs. The antibodies are of the IgG1 κ-type and appear to be directed against a conformation-dependent epitope in the D-domain of fibrin. Experiments with 99mTc-labeled Y22 in vitro show that Y22 binds rapidly to forming clots. 99mTc-Y22 also binds to performed plasma clots in a plasma milieu, even in the presence of high concentrations of heparin. Clot localization experiments in rabbits and rats confirm the high fibrin specificity and the potential of 99mTc-Y22 for thrombus imaging in vivo. Chemicals/CAS: fibrin, 9001-31-4; technetium 99m, 14133-76-7; Antibodies, Monoclonal; Binding Sites, Antibody; Fibrin, 9001-31-4; Fibrinogen, 9001-32-5; Technetium, 7440-26-8
Subject
Biology
Fibrinogen degradation product
Monoclonal antibody
Technetium 99m
Animal cell
Human
Human cell
Immunoscintigraphy
Mouse
Nonhuman
Normal human
Thrombosis
Abdomen
Animal
Antibodies, Monoclonal
Binding Sites, Antibody
Fibrin
Fibrinogen
Immunoenzyme Techniques
Jugular Veins
Male
Mice
Mice, Inbred BALB C
Rabbits
Rats
Support, Non-U.S. Gov't
Technetium
Thrombosis
Vena Cava, Inferior
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TNO identifier
230780
ISSN
0006-4971
Source
Blood, 74 (2), 708-714
Document type
article