Print Email Facebook Twitter An antifibrin monoclonal antibody useful in immunoscintigraphic detection of thrombi Title An antifibrin monoclonal antibody useful in immunoscintigraphic detection of thrombi Author Wasser, M.N.J.M. Koppert, P.W. Arndt, J.W. Emeis, J.J. Feitsma, R.I.J. Pauwels, E.K.J. Nieuwenhuizen, W. Gaubius Instituut TNO Publication year 1989 Abstract Balb/c mice were immunized with human plasmin-generated fibrinogen degradation product Y. Spleen cells were fused with P3X63-Ag8.653 myeloma cells. A clone (Y22) was found that produces monoclonal antibodies (MoAbs) with a strong reactivity with human fibrin and only a weak reactivity with fibrinogen in an enzyme immunoassay (EIA). Y22 also reacts with fibrin of rabbits, rats, sheep, and dogs. The antibodies are of the IgG1 κ-type and appear to be directed against a conformation-dependent epitope in the D-domain of fibrin. Experiments with 99mTc-labeled Y22 in vitro show that Y22 binds rapidly to forming clots. 99mTc-Y22 also binds to performed plasma clots in a plasma milieu, even in the presence of high concentrations of heparin. Clot localization experiments in rabbits and rats confirm the high fibrin specificity and the potential of 99mTc-Y22 for thrombus imaging in vivo. Chemicals/CAS: fibrin, 9001-31-4; technetium 99m, 14133-76-7; Antibodies, Monoclonal; Binding Sites, Antibody; Fibrin, 9001-31-4; Fibrinogen, 9001-32-5; Technetium, 7440-26-8 Subject BiologyFibrinogen degradation productMonoclonal antibodyTechnetium 99mAnimal cellHumanHuman cellImmunoscintigraphyMouseNonhumanNormal humanThrombosisAbdomenAnimalAntibodies, MonoclonalBinding Sites, AntibodyFibrinFibrinogenImmunoenzyme TechniquesJugular VeinsMaleMiceMice, Inbred BALB CRabbitsRatsSupport, Non-U.S. Gov'tTechnetiumThrombosisVena Cava, Inferior To reference this document use: http://resolver.tudelft.nl/uuid:70f8515b-eb56-42ab-8ade-22c5faa0df91 TNO identifier 230780 ISSN 0006-4971 Source Blood, 74 (2), 708-714 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.