Print Email Facebook Twitter Fluorometric determination of retinol in human tear fluid using high-performance liquid chromatography Title Fluorometric determination of retinol in human tear fluid using high-performance liquid chromatography Author Speek, A.J. van Agtmaal, E.J. Saowakontha, S. Schreurs, W.H.P. van Haeringen, N.J. Centraal Instituut voor Voedingsonderzoek TNO Publication year 1986 Abstract A fast and sensitive high-performance liquid chromatographic (HPLC) method for the determination of vitamin A (all-trans retinol) in 50 ??l human tear fluid is described. After deproteinization with ethanol and extraction with n-hexane, retinol is separated from the extract on a straight-phase HPLC column and detected fluorometrically. Vitamin A can be determined in concentrations as low as 0.4 ng/ml. A single analysis can be completed in 12 min while the analysis of a series of 60 samples takes about 8 h. The within-assay and between-assay coefficients of variation were 3.1 and 4.2% resp. The between-assay analytical recovery of retinol added to tear fluid was 97.4 ?? 6.0% (mean ?? SD). Retinol was determined in tear fluid of nine adult volunteers. The amounts observed ranged from <0.4-10.6 ng/ml. Chemicals/CAS: retinol, 68-26-8, 82445-97-4; Vitamin A, 11103-57-4 Subject RetinolDrug analysisDrug determinationDrug tissue levelFluorometryHigh performance liquid chromatographyHumanHuman cellMethodologyNormal humanTearVisual systemChromatography, High Pressure LiquidDrug StabilityEvaluation StudiesFluorometryHumanSupport, Non-U.S. Gov'tTearsVitamin ABiologyBiochemistryMedicineGeneeskunde To reference this document use: http://resolver.tudelft.nl/uuid:70120145-972f-4e7a-8d15-65e1b0e1594d TNO identifier 230190 ISSN 0271-3683 Source Current Eye Research, 5 (11), 841-845 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.