Title
An upstream activating sequence from the Aspergillus nidulans gpdA gene
Author
Punt, P.J.
Kramer, C.
Kuyvenhoven, A.
Pouwels, P.H.
van den Hondel, C.A.M.J.J.
Medisch Biologisch Laboratorium TNO
Publication year
1992
Abstract
Introduction of a previously identified promoter element of the Aspergillus nidulans gpdA gene (encoding glyceraldehyde-3-phosphate dehydrogenase), the so-called gpd box, into the upstream region of the highly regulated A. nidulans amdS gene (encoding acetamidase), significantly increased (up to 30-fold) the expression of the lacZ reporter gene fused to these expression signals. This increase was dependent on the orientation of the gpd box and on the site of introduction into the amdS upstream region. The presence of additional gpdA sequences which flank the gpd box reduced or even extinguished positive effects of the gpd box. ω-Amino acid and carbon catabolite regulation of the amdS promoter were retained after introduction of the gpd box, indicating that the gpd box does not abolish interactions of the regulatory proteins, AmdR and CreA, with the amdS transcription control sequences. Based on these results, it is suggested that the gpd box comprises at least two separate activities: one being orientation dependent, but relatively independent of position of the gpd box in the upstream region, and the other is only functional near other sites of transcripitonal control. Most likely, both activities are not involved in regulation of the amdS promoter.
Subject
ω-Amino acid induction
amdS::lacZ fusion gene
Carbon catabolite repression
Nitrogen metabolite repression
Recombinant DNA
Targeted single-copy integration
Carbon
Nitrogen
Aspergillus nidulans
Catabolism
Gene expression
Genetic regulation
Nonhuman
Nucleotide sequence
Priority journal
Promoter region
Reporter gene
Aspergillus nidulans
Base Sequence
Gene Expression Regulation, Fungal
Genetic Vectors
Glyceraldehyde-3-Phosphate Dehydrogenases
Molecular Sequence Data
Promoter Regions (Genetics)
Regulatory Sequences, Nucleic Acid
Aspergillus
Emericella nidulans
To reference this document use:
http://resolver.tudelft.nl/uuid:6f1f1762-7cc3-47fa-950a-0c6ded3b9cac
DOI
https://doi.org/10.1016/0378-1119(92)90010-m
TNO identifier
231951
ISSN
0378-1119
Source
Gene, 120 (1), 67-73
Bibliographical note
Correspondence Address: Punt, P.J.; Medical Biological Laboratory, TNO, P.O. Box 45, 2280 AA Rijswijk, Netherlands Chemicals/CAS: Genetic Vectors; Glyceraldehyde-3-Phosphate Dehydrogenases, EC 1.2.1.-
Document type
article