Title
Target recognition and synapse formation by ciliary-ganglion neurons in tissue culture
Author
Stevens, W.F.
Slaaf, D.W.
Hooisma, J.
Magchielse, T.
Meeter, E.
Medisch Biologisch Laboratorium TNO
Publication year
1978
Abstract
A less complicated source of neurons suitable for this type of studies is the parasympathetic ciliary ganglion. In the pigeon and in the chick this ganglion is known to contain only two classes of neurons, both of which are cholinoceptive and cholinergic and that innervate the muscle fibres of the choroid plexus of the eye, the sphincter iridis and the ciliary body (Marwitt et al., 1971). The iris and the ciliary muscle in birds are both striated (Ovio, 1927), whereas in the mammal they are smooth. Neurons of the ciliary ganglion of the chick embryo can be cultivated in tissue culture (Hooisma et al., 1975) either in explants of whole ganglia or after dissociation, as individual cells. Branching neurites grow radially from the ganglia. A corona of neurites with a diameter of 500-1000 μm surrounding the ganglia is formed during 2 days in culture. Mixed cultures of ciliary neurons and skeletal-muscle cells have been used for the study of the process of neuron-to-target recognition.
Subject
Biology
Animal experiment
Chicken
Ciliary ganglion
Embryo
In vitro study
Innervation
Muscle
Ontogeny
Peripheral nervous system
Tissue culture
Animal
Cell Communication
Cell Fusion
Chick Embryo
Ganglia, Autonomic
Muscles
Neurons
Synapses
Tissue Culture
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http://resolver.tudelft.nl/uuid:6dd0da47-7582-4028-9e36-75d97908e044
TNO identifier
228414
ISSN
0300-5127
Source
Biochemical Society Transactions, 6 (3), 487-490
Document type
article