Print Email Facebook Twitter 1H-NMR spectroscopy combined with pattern recognition analysis reveals characteristic chemical patterns in urines of MS patients and non-human primates with MS-like disease Title 1H-NMR spectroscopy combined with pattern recognition analysis reveals characteristic chemical patterns in urines of MS patients and non-human primates with MS-like disease Author 't Hart, B.A. Vogels, J.T.W.E. Spijksma, G. Brok, H.P.M. Polman, C. van der Greef, J. Primatencentrum TNO Publication year 2003 Abstract Proton nuclear magnetic resonance (1H-NMR) spectroscopy in combination with pattern recognition techniques were used to investigate the composition of organic compounds in urines from patients with multiple sclerosis (MS), patients with other neurological diseases (OND) and healthy controls (H). Using a valid animal model of MS, namely the common marmoset (Callithrix jacchus) model of experimental autoimmune encephalomyelitis (EAE), the relation of disease progression and alteration of the urine composition was investigated. Urine samples were collected during different stages of EAE, either induced with whole human myelin or with the myelin protein MOG in complete adjuvant. The urine samples were analysed with 1H-NMR spectroscopy allowing simultaneous detection of an array of compounds. Spectral differences between urines from EAE-affected and healthy monkeys were assessed with multivariate analysis. Evidence is provided that development of EAE is associated with changes in the chemical composition of the urine, in particular of compounds with NMR peaks in the region of the spectrum between 0.5 and 3.50 ppm. In addition, we found preliminary evidence for differences between urines from MS, OND and H groups. © 2003 Elsevier Science B.V. All rights reserved. Chemicals/CAS: tritium, 10028-17-8; Glycoproteins; Myelin Proteins; Tritium, 10028-17-8 Subject BiologyAnalytical researchInflammatory demyelinationMS disease markersNMR spectroscopyPattern recognitionUrinemyelin proteinorganic compoundglycoproteintritiumanimal modelarticleautoimmune diseasechemical compositionclinical articlecontrolled studydisease courseencephalomyelitisfemalehumanmalemarmosetmolecular recognitionmultiple sclerosismultivariate analysisnonhumanprimatepriority journalproton nuclear magnetic resonanceurinalysisvalidation processallergic encephalomyelitisanimalautomated pattern recognitionchemistrycomparative studydisease modelimmunizationimmunologymethodologynuclear magnetic resonance spectroscopyspecies differencetimeurineAnimalsCallithrixDisease Models, AnimalEncephalomyelitis, Autoimmune, ExperimentalFemaleGlycoproteinsHumansImmunizationMagnetic Resonance SpectroscopyMaleMultiple SclerosisMultivariate AnalysisMyelin ProteinsPattern Recognition, AutomatedSpecies SpecificityTime FactorsTritiumUrine To reference this document use: http://resolver.tudelft.nl/uuid:6dab44c8-2e31-4fe6-99d3-d27c600c3e08 DOI https://doi.org/10.1016/s0022-510x(03)00080-7 TNO identifier 237225 ISSN 0022-510X Source Journal of the Neurological Sciences, 212 (1-2), 21-30 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.