Title
Automated electron tomography of the septal pore cap in Rhizoctonia solani
Author
Müller, W.H.
Koster, A.J.
Humbel, B.M.
Ziese, U.
Verkleij, A.J.
van Aelst, A.C.
van der Krift, T.P.
Montijn, R.C.
Boekhout, T.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
2000
Abstract
Dolipore septa and septal pore caps (SPCs) in filamentous basidiomycetes may play an important role in maintaining the integrity of hypal cells. We have investigated the ultrastructure of the dolipore septum and the SPC in Rhizoctonia solani hyphal cells after high-pressure freezing, freeze substitution, and Spurr embedding. We visualized the SPC with associated cell ultrastructures in three dimensions by automated electron tomography of thick-sectioned cells, followed by 3D tomographic reconstructions. Using these methods we were able to document the passage of mitochondria through the SPC, small tubular membranous structures at the entrance of the septal pore channel, filamentous structures connecting the inner side of the SPC with pore-plugging material, thin filaments anchoring the pore-plugging material with the plasma membrane, small vesicles attached to the plugging material, and tubular endoplasmic reticulum continuous with the base of the SPC. We hypothesize that the SPC, the filamentous structures, the plugging material, and the endoplasmic reticulum act in a coordinated fashion to maintain cellular integrity, intercellular communication, and the transport of solutes and cell organelles in the filamentous fungus R. solani. (C) 2000 Academic Press.
Subject
Nutrition
Botany
Fungi
β-1,6-glucan
Cryo-scanning electron microscopy
Dolipore septum
Electron tomography
Filamentous structures
Freeze substitution
Fungus
High-pressure freezing
Rhizoctonia solani
Septal pore cap
Transmission electron microscopy
ZIO staining
Automation
Cell communication
Cell ultrastructure
Channel gating
Diagnostic value
Electron beam tomography
Hyphomycetes
Membrane structure
Nonhuman
Priority journal
Automation
Cell Membrane
Cryoelectron Microscopy
Electrons
Endoplasmic Reticulum
Freezing
Image Processing, Computer-Assisted
Intercellular Junctions
Intracellular Membranes
Microscopy, Electron
Microscopy, Electron, Scanning
Mitochondria
Rhizoctonia
Tomography
Basidiomycota
Fungi
Hyphomycetes
Rhizoctonia
Thanatephorus cucumeris
To reference this document use:
http://resolver.tudelft.nl/uuid:6cc03ba7-569f-4c7d-95d4-d09b3378f8a6
DOI
https://doi.org/10.1006/jsbi.2000.4243
TNO identifier
72003
Source
Journal of Structural Biology, 131 (1), 10-18
Document type
article