Title
Streptococcus salivarius MS-oral-D6 promotes gingival re-epithelialization in vitro through a secreted serine protease
Author
Fernandez-Gutierrez, M.M.
Roosjen, P.P.J.
Ultee, E.
Agelink, M.
Vervoort, J.J.M.
Keijser, B.
Wells, J.M.
Kleerebezem, M.
Publication year
2017
Abstract
Gingival re-epithelialization represents an essential phase of oral wound healing in which epithelial integrity is re-establish. We developed an automated high-throughput re-epithelialization kinetic model, using the gingival epithelial cell line Ca9-22. The model was employed to screen 39 lactic acid bacteria, predominantly including oral isolates, for their capacity to accelerate gingival re-epithelialization. This screen identified several strains of Streptococcus salivarius that stimulated re-epithelialization. Further analysis revealed that S. salivarius strain MS-oral-D6 significantly promoted re-epithelialization through a secreted proteinaceous compound and subsequent experiments identified a secreted serine protease as the most likely candidate to be involved in re-epithelialization stimulation. The identification of bacteria or their products that stimulate gingival wound repair may inspire novel strategies for the maintenance of oral health. © 2017 The Author(s).
Subject
ELSS - Earth, Life and Social Sciences
Life
Healthy Living
Biomedical Innovation
Biology
MSB - Microbiology and Systems Biology
To reference this document use:
http://resolver.tudelft.nl/uuid:6c0fa032-b983-496f-b066-e6b4c4f57936
DOI
https://doi.org/10.1038/s41598-017-11446-z
TNO identifier
781376
ISSN
2045-2322
Source
Scientific Reports, 7 (7)
Document type
article