Print Email Facebook Twitter Protein synthesis inhibition by cycloheximide does not affect the acute release of tissue-type plasminogen activator Title Protein synthesis inhibition by cycloheximide does not affect the acute release of tissue-type plasminogen activator Author Tranquille, N. Emeis, J.J. Gaubius Instituut TNO Publication year 1989 Abstract The acute release of tissue-type plasminogen activator (t-PA) was studied in perfused rat hindlegs. Pretreatment of rats with the protein synthesis inhibitor cycloheximide (2 mg/kg) at 1, 3 or 5 h prior to perfusion of rat hindlegs did not influence the amount of t-PA released by platelet-activating factor (20 nM) or bradykinin (1 μM). The amount of t-PA activity that could be extracted from hindleg skeletal muscle was not decreased by cycloheximide pretreatment though it was decreased in lung extracts. The in vivo release of t-PA was not affected by cycloheximide pretreatment. The data suggest that the acute release of t-PA from vascular endothelial cells does not require ongoing protein synthesis, but that acutely released t-PA is derived from a stable endothelial storage pool. Chemicals/CAS: cycloheximide, 642-81-9, 66-81-9; plasminogen activator, 9039-53-6; Cycloheximide, 66-81-9; Protein Synthesis Inhibitors; Tissue Plasminogen Activator, EC 22.214.171.124 Subject Healthcycloheximideplasminogen activatoranimal cellenzyme inhibitionnonhumanAnimalCycloheximideFibrinolysisHindlimbLungMaleMusclesPerfusionProtein Synthesis InhibitorsRatsRats, Inbred StrainsTissue Plasminogen Activator To reference this document use: http://resolver.tudelft.nl/uuid:6c007735-1357-44f3-af1e-44d5cf192b87 TNO identifier 230886 ISSN 0340-6245 Source Thrombosis and Haemostasis, 61 (3), 442-447 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.