Title
Detection of zilpaterol (Zilmax®) in calf urine and faeces with liquid chromatography-tandem mass spectrometry
Author
van Hoof, N.
Schilt, R.
van der Vlis, E.
Boshuis, P.
van Baak, M.
Draaijer, A.
de Wasch, K.
van de Wiele, M.
van Hende, J.V.
Courtheyn, D.
de Brabander, H.
TNO Kwaliteit van Leven
Publication year
2005
Abstract
Zilpaterol is a new powerful beta-agonist, which is officially registered for fattening purposes in cattle in Mexico and South Africa. Its chemical structure is different from the well-known beta-agonists. Therefore, the routinely used screening methods are not likely to be suited for the analysis of zilpaterol. Also gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry (LC-MS) methods need to be adapted to enable detection of zilpaterol. In this study, a LC-MS3 confirmatory method was developed for the simultaneous detection of zilpaterol and di-aromatic beta-agonists in urine samples. A LC-MS2 method was optimised for the detection of zilpaterol in faeces. To study the excretion profile in urine and faeces, a male veal calf was orally treated with daily doses of Zilmax ® during 2 weeks. Zilpaterol was mainly excreted via urine. © 2004 Elsevier B.V. All rights reserved.
Subject
Toxicology
Analytical research
Beta-agonists
Excretion profile
LC-MSn
Zilpaterol
Body fluids
Liquid chromatography
Mass spectrometry
Optimization
Calf urine
Faeces
Fattening
Zilpaterol
Alcohols
beta adrenergic receptor stimulating agent
unclassified drug
zilmax
zilpaterol
animal experiment
animal tissue
cattle
conference paper
controlled study
drug determination
drug excretion
drug structure
gas chromatography
liquid chromatography
male
mass spectrometry
nonhuman
priority journal
tandem mass spectrometry
urinalysis
Bos taurus
To reference this document use:
http://resolver.tudelft.nl/uuid:6b7a94db-d936-4b12-951d-2cd4d3ea074b
DOI
https://doi.org/10.1016/j.aca.2004.08.072
TNO identifier
238312
ISSN
0003-2670
Source
Analytica Chimica Acta, 529 (1-2 SPEC. ISS.), 189-197
Document type
article