Title
Agrobacterium-mediated transformation as a tool for functional genomics in fungi
Author
Michielse, C.B.
Hooykaas, P.J.J.
van den Hondel, C.A.M.J.J.
Ram, A.F.J.
TNO Kwaliteit van Leven
Publication year
2005
Abstract
In the era of functional genomics, the need for tools to perform large-scale targeted and random mutagenesis is increasing. A potential tool is Agrobacterium-mediated fungal transformation. A. tumefaciens is able to transfer a part of its DNA (transferred DNA; T-DNA) to a wide variety of fungi and the number of fungi that can be transformed by Agrobacterium-mediated transformation (AMT) is still increasing. AMT has especially opened the field of molecular genetics for fungi that were difficult to transform with traditional methods or for which the traditional protocols failed to yield stable DNA integration. Because of the simplicity and efficiency of transformation via A. tumefaciens, it is relatively easy to generate a large number of stable transformants. In combination with the finding that the T-DNA integrates randomly and predominantly as a single copy, AMT is well suited to perform insertional mutagenesis in fungi. In addition, in various gene-targeting experiments, high homologous recombination frequencies were obtained, indicating that the T-DNA is also a useful substrate for targeted mutagenesis. In this review, we discuss the potential of the Agrobacterium DNA transfer system to be used as a tool for targeted and random mutagenesis in fungi. © Springer-Verlag 2005.
Subject
Biology
Analytical research
Agrobacterium tumefaciens
Fungal transformation
Insertional mutagenesis
Targeted mutagenesis
bacterial DNA
DNA integration
DNA synthesis
DNA transfer
functional genomics
fungal genetics
fungal strain
gene expression regulation
gene targeting
genetic transformation
molecular genetics
nonhuman
priority journal
review
Rhizobium
Rhizobium radiobacter
Bacterial Proteins
Base Sequence
DNA, Fungal
Genome, Fungal
Genomics
Mutagenesis, Insertional
Mutagenesis, Site-Directed
Plasmids
Rhizobium
Saccharomyces cerevisiae
Agrobacterium
Agrobacterium tumefaciens
Fungi
To reference this document use:
http://resolver.tudelft.nl/uuid:6b053f52-c850-425c-a6a3-1849f3928f23
DOI
https://doi.org/10.1007/s00294-005-0578-0
TNO identifier
238591
ISSN
0172-8083
Source
Current Genetics, 48 (1), 1-17
Document type
article