Title
Abscisic acid induces a cytosolic calcium decrease in barley aleurone protoplasts
Author
Instituut voor Biotechnologie en Chemie TNO
Wang, M.
van Duijn, B.
Schram, A.W.
Publication year
1991
Abstract
Cytosolic calcium concentrations (Ca(i)) of barley aleurone protoplasts after stimulation with the plant hormone abscisic acid (ABA) were measured by using the calcium-sensitive fluorescent dye Indo-1. The measuredd basal Ca(i) is about 200 nM. Stimulation with ABA induces a strong dose-dependent decrease in Ca(i) to a minimal value of about 50 nM. This decrease occurs within 5 s. The Ca2+ antagonists La3+ and Cd2+ inhibit the ABA-induced Ca(i) decrease in a dose-dependent manner, while the Ca2+ channel blockers verapamil and nifedipine give no inhibition. The induction of Ca(i) decrease by ABA is consistent with activation of the plasma membrane Ca2+-ATPase by ABA. The possible role of this ABA-induced Ca(i) decrease in ABA signal transduction and in counteracting the effects of gibberellic acid are discussed. Chemicals/CAS: Histocompatibility Antigens Class IIChemicals/CAS: Abscisic Acid, 21293-29-8; Ca(2+)-Transporting ATPase, EC 3.6.1.38; Calcium, 7440-70-2; Fluorescent Dyes; indo-1, 96314-96-4; Indoles; Nifedipine, 21829-25-4; Verapamil, 52-53-9
Subject
Barley aleurone
Dormancy
Gibberellic acid
Cadmium
Lanthanide
Plant
Abscisic Acid
Ca(2+)-Transporting ATPase
Calcium
Cell Membrane
Cytosol
Enzyme Activation
Fluorescent Dyes
Hordeum
Indoles
Nifedipine
Protoplasts
Support, Non-U.S. Gov't
Verapamil
Hordeum vulgare subsp. vulgare
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DOI
https://doi.org/10.1016/0014-5793(91)80086-i
TNO identifier
231480
ISSN
0014-5793
Source
FEBS Letters, 278 (278), 69-74
Document type
article