Title
Infection of a canine macrophage cell line with Leishmania infantum: Determination of nitric oxide production and anti-leishmanial activity
Author
Pinelli, E.
Gebhard, D.
Mommaas, A.M.
van Hoeij, M.
Langermans, J.A.M.
Ruitenberg, E.J.
Rutten, V.P.M.G.
Publication year
2000
Abstract
We have previously shown that resistance to Leishmania infantum in dogs is associated with a Th1 type of immune response. In this study, we use a canine macrophage cell line (030-D) that can readily be infected with this protozoan parasite. Our aim is to further characterize the effector mechanisms involved in killing of Leishmania parasite in dogs. We observed that activation of 030-D cells by incubation with a supernatant derived from a Leishmania-specific T cell line containing IFN-γ, TNF-α and interleukin-2 (IL-2) resulted in enhanced nitric oxide (NO) production by these cells. In addition, we observed enhanced anti-leishmanial activity of infected 030-cells after activation. Both, NO production and anti-leishmanial activity were abrogated by addition of L-N(G)-nitroargininemethyl ester (L-NAME), an analogue of L-arginine. Thus, NO play an important role in the anti-leishmanial activity of these canine macrophages. We propose the infection of the 030-D cell line as a good in vitro model to further investigate parasite-host cell interactions in dogs, a natural host of Leishmania parasites. Copyright (C) 2000 Elsevier Science B.V.
Subject
Dog
Leishmania infantum
Macrophage cell line
Cytokine
Interleukin 2
Nitric oxide
Animal cell
Animal experiment
Cell line
Host parasite interaction
In vitro study
Parasite cultivation
Promastigote
T lymphocyte
Animals
Cell Line
Dog Diseases
Enzyme Inhibitors
Interferon Type II
Leishmania infantum
Leishmaniasis, Visceral
Macrophage activation
Macrophages
Microscopy, Electron
NG-Nitroarginine Methyl Ester
Nitric Oxide
Canis familiaris
Protozoa
Arginine, 74-79-3
Enzyme Inhibitors
Interferon Type II, 82115-62-6
Interleukin-2
NG-Nitroarginine methyl ester, 50903-99-6
Nitric Oxide, 10102-43-9
Tumor necrosis factor-alpha
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DOI
https://doi.org/10.1016/s0304-4017(00)00312-5
TNO identifier
235692
ISSN
0304-4017
Source
Veterinary Parasitology, 92 (92), 181-189
Document type
article