Print Email Facebook Twitter The effects of the fibrin-derived peptide Bβ15-42 in acute and chronic rodent models of myocardial ischemia-reperfusion Title The effects of the fibrin-derived peptide Bβ15-42 in acute and chronic rodent models of myocardial ischemia-reperfusion Author Zacharowski, K. Zacharowski, P.A. Friedl, P. Mastan, P. Koch, A. Boehm, O. Rother, R.P. Reingruber, S. Henning, R. Emeis, J.J. Petzelbauer, P. TNO Kwaliteit van Leven Publication year 2007 Abstract Many compounds have been shown to prevent reperfusion injury in various animal models, although to date, translation into clinic has revealed several obstacles. Therefore, the National Heart, Lung, and Blood Institute convened a working group to discuss reasons for such failure. As a result, the concept of adequately powered, blinded, randomized studies for preclinical development of a compound has been urged. We investigated the effects of a fibrin-derived peptide Bβ15-42 in acute and chronic rodent models of ischemia-reperfusion at three different study centers (Universities of Dusseldorf and Vienna, TNO Biomedical Research). A total of 187 animals were used, and the peptide was compared with the free radical scavenger Tempol, CD18 antibody, α-C5 antibody, and the golden standard, ischemic preconditioning. We show that Bβ15-42 robustly and reproducibly reduced infarct size in all models of ischemia-reperfusion. Moreover, the peptide significantly reduced plasma levels of the cytokines interleukin 1β, tumor necrosis factor α, and interleukin 6. In rodents, Bβ15-42 inhibits proinflammatory cytokine release and is cardioprotective during ischemia-reperfusion injury. ©2007The Shock Society. Subject BiologyBiomedical ResearchFibrin-derived peptideIschemia-reperfusionMyocardial infarctionPreclinical study centersCD18 antigenComplement component C5 inhibitorFibrin derivativePeptide derivativeScavengerTempolAcute heart infarctionAnimal experimentAnimal modelAnimal tissueArticleControlled studyCytokine releaseDrug effectHeart infarction sizeHeart muscle ischemiaHeart muscle reperfusionHeart protectionIschemic preconditioningLeukocyte migrationMaleNonhumanRatSingle drug doseTherapy effectTreatment outcomeTreatment responseAnimalsAntigens, CD18Cyclic N-OxidesDisease Models, AnimalFibrinogenFree Radical ScavengersInterleukin-6MiceMice, Inbred BALB CModels, BiologicalMyocardial InfarctionMyocardial ReperfusionMyocardiumRatsReperfusion InjurySpin Labels To reference this document use: http://resolver.tudelft.nl/uuid:680abf3c-dab3-4867-a0c3-a297a2a73a37 DOI https://doi.org/10.1097/shk.0b013e31802fa038 TNO identifier 240024 ISSN 1073-2322 Source Shock, 27 (6), 631-637 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.