Print Email Facebook Twitter Neonatal supplementation of processed supernatant from Lactobacillus rhamnosus GG improves allergic airway inflammation in mice later in life Title Neonatal supplementation of processed supernatant from Lactobacillus rhamnosus GG improves allergic airway inflammation in mice later in life Author Harb, H. van Tol, E.A.F. Heine, H. Braaksma, M. Gross, G. Overkamp, K. Hennen, M. Alrifai, M. Conrad, M.L. Renz, H. Garn, H. Publication year 2013 Abstract Background: Oral supplementation with probiotic bacteria can protect against the development of allergic and inflammatory diseases. Objective: The aim of this study was to investigate potential immunomodulatory and allergy-protective effects of processed Lactobacillus rhamnosus GG (LGG)-derived supernatants early in life in neonatal mice. Methods: In vitro, RAW264.7 mouse macrophages were stimulated with viable LGG, LGG-derived supernatants, prepared from different growth phases, and different size fractions thereof, and pro- and anti-inflammatory cytokine production was analysed. Supernatant fractions were also treated with protease, DNAse or carbohydrate-digesting enzymes to define the nature of immunomodulatory components. In vivo, neonatal Balb/c mice were orally supplemented with differentially processed LGG supernatants. Starting at 4 weeks of age, a protocol of ovalbumin-induced acute allergic airway inflammation was applied and protective effects of processed LGG supernatants were assessed. Results: Incubation of RAW264.7 cells with LGG-derived supernatants significantly increased TNFα and IL-10 production. These effects were not restricted to a particular molecular size fraction. Treatment with protease, but not with DNAse or carbohydrate-digesting enzymes, completely abolished the immunomodulatory activities. Incubation of TLR/NOD-transfected cells with LGG-derived supernatants revealed that recognition and signalling of bioactive components is mediated by TLR2 and NOD2. In vivo supplementation of newborn mice with processed LGG-derived supernatants resulted in pronounced protective effects on the allergic inflammatory response as reflected by reduced eosinophil numbers, modified T helper cell cytokine production, significantly less lung inflammation and reduced goblet cell numbers in comparison with sham-treated controls. Conclusion: LGG-derived supernatants exert immunomodulatory activities, and neonatal administration of specifically processed supernatants may provide an alternative to viable probiotics in reducing allergic inflammatory responses. © 2012 Blackwell Publishing Ltd. Subject LifeMSB - Microbiology and Systems BiologyEELS - Earth, Environmental and Life SciencesBiomedical InnovationBiologyHealthy LivingAsthmaLGGNeonatal Animal ModelPreventionSoluble FactorsInterleukin 10Probiotic AgentTumor Necrosis Factor AlphaAllergic Airway InflammationAnimal CellAnimal ExperimentArticleControlled StudyCytokine ProductionfemaleHelper CellImmunomodulationIn Vitro StudyIn Vivo StudyLactobacillus RhamnosusmacrophageMouseNewbornNonhuman To reference this document use: http://resolver.tudelft.nl/uuid:671f788b-5dc7-4437-b9f9-c72047e880f1 DOI https://doi.org/10.1111/cea.12047 TNO identifier 471481 ISSN 0954-7894 Source Clinical and Experimental Allergy, 43 (3), 353-364 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.