Print Email Facebook Twitter Intestinal T lymphocytes of different rat strains in immunotoxicity Title Intestinal T lymphocytes of different rat strains in immunotoxicity Author Bruder, M.C. Spanhaak, S. Bruijntjes, J.P. Michielsen, C.P.P.C. Vos, J.G. Kuper, C.F. Centraal Instituut voor Voedingsonderzoek TNO Publication year 1999 Abstract In order to study the intestinal mucosal immune cells, with emphasis on single T lymphocytcs, an inventory was made of single and organized lymphocytes in the epithelium and lamina propria of the small intestines of untreated Wistar, Fischer 344, and Lewis rats. The single and organized lymphocytes were examined microscopically. In addition, the single lymphocytes in the epithelium tIEL) and lamina propria (LPL) were analyzed by flow cytometry. Next, the use of flow cytometry analysis was explored to detect changes in the IEL T-lymphocyte population in subacute oral studies with the immunomodulating agents azathioprine and hexachlorobenzene. Untreated random-bred Wistar rats exhibited a large interindividual variability in IEL composition, while the variability was small in inbred Fischer 344 and Lewis rats. The explorative study with the 2 model immunomodulating compounds demonstrated that hexachlorobenzene increased the number of intraepithelial T lymphocytes with CD8+ phenotype at the cost oft cells with CD4+ phenotype in Lewis rats. Azathioprine did not induce distinct effects on the percentages of IEL. The data indicate that the intraepithelial lymphocytes in the intestines are a potential target for orally administered immunomodulating compounds and should therefore receive more attention in toxicologic pathology studies. Subject NutritionAzathioprineGut mucosal immunityHexachlorobenzeneIntraepithelial lymphocytesLamina propria lymphocytesAzathioprineHexachlorobenzeneAnimal experimentBlood toxicityCell countCell isolationControlled studyDrug effectDrug targetingFemaleFlow cytometryGastrointestinal toxicityImmunomodulationImmunotoxicityIntestine epitheliumIntestine mucosaMaleNonhumanOral drug administrationPriority journalRatT lymphocyteAnimalsAzathioprineBody WeightCD4-CD8 RatioCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesFemaleFlow CytometryHexachlorobenzeneImmunohistochemistryImmunosuppressive AgentsIntestinal MucosaIntestinesKiller Cells, NaturalLymphocyte CountMaleRatsRats, Inbred F344Rats, Inbred LewRats, WistarReceptors, Antigen, T-Cell, gamma-deltaSpecies SpecificityT-LymphocytesAnimaliaRattus norvegicus To reference this document use: http://resolver.tudelft.nl/uuid:663839a6-e6a4-47ec-8b7d-99f72d16316e TNO identifier 235005 ISSN 0192-6233 Source Toxicologic Pathology, 27 (2), 171-179 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.