Title
Intestinal T lymphocytes of different rat strains in immunotoxicity
Author
Bruder, M.C.
Spanhaak, S.
Bruijntjes, J.P.
Michielsen, C.P.P.C.
Vos, J.G.
Kuper, C.F.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
1999
Abstract
In order to study the intestinal mucosal immune cells, with emphasis on single T lymphocytcs, an inventory was made of single and organized lymphocytes in the epithelium and lamina propria of the small intestines of untreated Wistar, Fischer 344, and Lewis rats. The single and organized lymphocytes were examined microscopically. In addition, the single lymphocytes in the epithelium tIEL) and lamina propria (LPL) were analyzed by flow cytometry. Next, the use of flow cytometry analysis was explored to detect changes in the IEL T-lymphocyte population in subacute oral studies with the immunomodulating agents azathioprine and hexachlorobenzene. Untreated random-bred Wistar rats exhibited a large interindividual variability in IEL composition, while the variability was small in inbred Fischer 344 and Lewis rats. The explorative study with the 2 model immunomodulating compounds demonstrated that hexachlorobenzene increased the number of intraepithelial T lymphocytes with CD8+ phenotype at the cost oft cells with CD4+ phenotype in Lewis rats. Azathioprine did not induce distinct effects on the percentages of IEL. The data indicate that the intraepithelial lymphocytes in the intestines are a potential target for orally administered immunomodulating compounds and should therefore receive more attention in toxicologic pathology studies.
Subject
Nutrition
Azathioprine
Gut mucosal immunity
Hexachlorobenzene
Intraepithelial lymphocytes
Lamina propria lymphocytes
Azathioprine
Hexachlorobenzene
Animal experiment
Blood toxicity
Cell count
Cell isolation
Controlled study
Drug effect
Drug targeting
Female
Flow cytometry
Gastrointestinal toxicity
Immunomodulation
Immunotoxicity
Intestine epithelium
Intestine mucosa
Male
Nonhuman
Oral drug administration
Priority journal
Rat
T lymphocyte
Animals
Azathioprine
Body Weight
CD4-CD8 Ratio
CD4-Positive T-Lymphocytes
CD8-Positive T-Lymphocytes
Female
Flow Cytometry
Hexachlorobenzene
Immunohistochemistry
Immunosuppressive Agents
Intestinal Mucosa
Intestines
Killer Cells, Natural
Lymphocyte Count
Male
Rats
Rats, Inbred F344
Rats, Inbred Lew
Rats, Wistar
Receptors, Antigen, T-Cell, gamma-delta
Species Specificity
T-Lymphocytes
Animalia
Rattus norvegicus
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http://resolver.tudelft.nl/uuid:663839a6-e6a4-47ec-8b7d-99f72d16316e
TNO identifier
235005
ISSN
0192-6233
Source
Toxicologic Pathology, 27 (2), 171-179
Document type
article