Print Email Facebook Twitter Influence of inhibition of nitric oxide synthesis on cardiac function in the newborn lamb after hypoxic-ischemic injury Title Influence of inhibition of nitric oxide synthesis on cardiac function in the newborn lamb after hypoxic-ischemic injury Author Dorrepaal, C.A. van Bel, F. Steendijk, P. Shadid, M. van de Velde, E.T. Baan, J. TNO Preventie en Gezondheid Publication year 2000 Abstract The aim of the present study was to investigate the effect of immediate post-hypoxic-ischemic (HI) inhibition of nitric oxide synthesis by N(ω)- nitro-L-arginine (NLA) on cardiac function and reactive oxygen species production. Fifteen newborn lambs were subjected to severe HI. Upon resuscitation 5 received 10 mg NLA/kg, 4 40 mg NLA/kg and 6 a placebo. Left ventricular (LV) contractility, cardiac output (CO), non-protein-bound iron (NPBI), ratio of reduced/oxidized ascorbic acid, α-tocopherol, sulfhydryl groups and malondialdehyde were measured before and 15, 60 and 120 min after resuscitation. There was a significant decrease in CO in all 3 groups at 60 min post-HI (p < 0.05). Reactive oxygen species production was also highest at 60 min post-HI (significantly increased NPBI and decrease in sulfhydryl groups in control lambs; p < 0.05). These results suggest neither a positive nor a negative effect of nitric oxide synthesis inhibition on post-HI myocardial performance but may suggest a positive effect of NLA on reactive oxygen species-mediated post-HI damage. Copyright (C) 2000 S. Karger AG, Basel. Chemicals/CAS: Enzyme Inhibitors; Nitric Oxide Synthase, EC 126.96.36.199; Nitroarginine, 2149-70-4; Reactive Oxygen Species Subject HealthFree radical productionHypoxia-ischemiaNitric oxideReperfusionVentricular functionAlpha tocopherolAscorbic acidIronIron binding proteinMalonaldehydeN(g) nitroarginineNitric oxideReactive oxygen metaboliteThiol groupAnimal experimentAnimal modelControlled studyheart functionHeart left ventricle contractilityHeart muscle ischemiaHeart muscle reperfusionHeart outputHeart performanceHeart ventricle functionLambNewbornNewborn hypoxiaNonhumanOxidation reduction stateOxidative stressReperfusion injuryAnimalsAnimals, NewbornCardiac OutputEnzyme InhibitorsHeartHeart VentriclesHypoxia-Ischemia, BrainMyocardial ContractionNitric Oxide SynthaseNitroarginineReactive Oxygen SpeciesSheepVenae CavaeOvis aries To reference this document use: http://resolver.tudelft.nl/uuid:65c24588-edd7-45ed-85da-62422f9f9c38 TNO identifier 235626 ISSN 0006-3126 Source Biology of the Neonate, 78 (2), 98-105 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.