Title
Influence of inhibition of nitric oxide synthesis on cardiac function in the newborn lamb after hypoxic-ischemic injury
Author
Dorrepaal, C.A.
van Bel, F.
Steendijk, P.
Shadid, M.
van de Velde, E.T.
Baan, J.
TNO Preventie en Gezondheid
Publication year
2000
Abstract
The aim of the present study was to investigate the effect of immediate post-hypoxic-ischemic (HI) inhibition of nitric oxide synthesis by N(ω)- nitro-L-arginine (NLA) on cardiac function and reactive oxygen species production. Fifteen newborn lambs were subjected to severe HI. Upon resuscitation 5 received 10 mg NLA/kg, 4 40 mg NLA/kg and 6 a placebo. Left ventricular (LV) contractility, cardiac output (CO), non-protein-bound iron (NPBI), ratio of reduced/oxidized ascorbic acid, α-tocopherol, sulfhydryl groups and malondialdehyde were measured before and 15, 60 and 120 min after resuscitation. There was a significant decrease in CO in all 3 groups at 60 min post-HI (p < 0.05). Reactive oxygen species production was also highest at 60 min post-HI (significantly increased NPBI and decrease in sulfhydryl groups in control lambs; p < 0.05). These results suggest neither a positive nor a negative effect of nitric oxide synthesis inhibition on post-HI myocardial performance but may suggest a positive effect of NLA on reactive oxygen species-mediated post-HI damage. Copyright (C) 2000 S. Karger AG, Basel. Chemicals/CAS: Enzyme Inhibitors; Nitric Oxide Synthase, EC 1.14.13.39; Nitroarginine, 2149-70-4; Reactive Oxygen Species
Subject
Health
Free radical production
Hypoxia-ischemia
Nitric oxide
Reperfusion
Ventricular function
Alpha tocopherol
Ascorbic acid
Iron
Iron binding protein
Malonaldehyde
N(g) nitroarginine
Nitric oxide
Reactive oxygen metabolite
Thiol group
Animal experiment
Animal model
Controlled study
heart function
Heart left ventricle contractility
Heart muscle ischemia
Heart muscle reperfusion
Heart output
Heart performance
Heart ventricle function
Lamb
Newborn
Newborn hypoxia
Nonhuman
Oxidation reduction state
Oxidative stress
Reperfusion injury
Animals
Animals, Newborn
Cardiac Output
Enzyme Inhibitors
Heart
Heart Ventricles
Hypoxia-Ischemia, Brain
Myocardial Contraction
Nitric Oxide Synthase
Nitroarginine
Reactive Oxygen Species
Sheep
Venae Cavae
Ovis aries
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http://resolver.tudelft.nl/uuid:65c24588-edd7-45ed-85da-62422f9f9c38
TNO identifier
235626
ISSN
0006-3126
Source
Biology of the Neonate, 78 (2), 98-105
Document type
article