In vitro proliferation of haemopoietic cells in the presence of adherent cell layers. I. Culture conditions and strain dependence

In vitro proliferation of haemopoietic cells in the presence of adherent cell layers. I. Culture conditions and strain dependence

Reimann, J.
Burger, H.
Radiobiologisch Instituut TNO

1979

The culture system, in which a marrow-derived adherent cell population, established in vitro, exerts a long-term promoting influence on proliferation of haemopoietic cells, is reproduced. Essential parameters of the system are investigated; it is confirmed that the system is critically dependent on horse serum, and on the in vitro age of the adherent cell layer. The growth-promoting effect on haemopoietic cells seems to be independent of the number of marrow cells per culture flask initially inoculated into the cultures to establish the adherent cell layer. In vitro established marrow-derived adherent cell layers from RFM (H-2(f)) and BALB/c (H-2(d)) mice can promote the long-term proliferation of syngeneic and allogeneic haemopoietic cells; haemopoietic marrow cells from C3H (H-2(k)) cannot be maintained on syngeneic or allogeneic (BALB/c, H-2(d)) adherent cell layers; adherent cell layers of C3H (H-2(k)) can maintain haemopoietic cells of the H-2(d) (BALB/c) genotype. This culture system does not reproduce the in vitro phenomenon of allogeneic resistance. The relevance of these findings to the suggestion that the growth-promoting activity of adherent marrow cells on haemopoietic stem cells in vitro duplicates aspects of the in vivo haemopoietic microenvironment is discussed.

Animal
Bone Marrow Cells
Cell Division
Cells, Cultured
Colony-Forming Units Assay
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Mice, Inbred Strains
Time Factors
Transplantation, Homologous
Transplantation, Isogeneic

http://resolver.tudelft.nl/uuid:649a74b6-e70e-494d-b7ce-1bbaf9aaadcb

228724

0301-472X

Experimental Hematology, 7 (1), 45-51

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