Title
Identification of secreted proteins of Aspergillus oryzae associated with growth on solid cereal substrates
Author
te Biesebeke, R.
Boussier, A.
van Biezen, N.
van den Hondel, C.A.M.J.J.
Punt, P.J.
TNO Kwaliteit van Leven
Publication year
2006
Abstract
Filamentous growth of Aspergillus oryzae on solid cereal substrates involves secretion of substrate converting enzymes and a solid substrate specific polarised hyphal growth phenotype. To identify proteins produced under these specific conditions, the extracts of A. oryzae grown on wheat-based media were analysed using N-terminal sequence analysis. In a submerged wheat-based growth medium of A. oryzae, besides α-amylase, also an arabinosidase and xylanase were abundantly produced. In the extracts of A. oryzae grown on wheat-based solid substrate besides α-amylase and chitinase, two new proteins of 16 and 27 kDa were identified. These hypothetical proteins showed only close homologies to filamentous fungal proteins. © 2005 Elsevier B.V. All rights reserved.
Subject
Biology
Biotechnology
Aspergillus oryzae
Cereal substrates
Filamentous
Cell culture
Fungi
Substrates
Aspergillus oryzae
Cereal substrates
Filamentous
Enzymes
alpha arabinofuranosidase
amylase
chitinase
fungal protein
xylan endo 1,3 beta xylosidase
amino terminal sequence
article
Aspergillus oryzae
cereal
culture medium
fungus growth
nonhuman
priority journal
protein analysis
protein secretion
sequence homology
Aspergillus oryzae
Cereals
Fungal Proteins
Aspergillus oryzae
Fungi
Triticum aestivum
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DOI
https://doi.org/10.1016/j.jbiotec.2005.08.028
TNO identifier
239134
ISSN
0168-1656
Source
Journal of Biotechnology, 121 (4), 482-485
Document type
article