Title
An enzymatic microreactor based on chaotic micromixing for enhanced amperometric detection in a continuous glucose monitoring application
Author
Moon, B.-U.
Koster, S.
Wientjes, K.J.C.
Kwapiszewski, R.M.
Schoonen, A.J.M.
Westerink, B.H.C.
Verpoorte, E.
TNO Kwaliteit van Leven
Publication year
2010
Abstract
The development of continuous glucose monitoring systems is a major trend in diabetes-related research. Small, easy-to-wear systems which are robust enough to function over many days without maintenance are the goal. We present a new sensing system for continuous glucose monitoring based on a microreactor incorporating chaotic mixing channels. Two different types of chaotic mixing channels with arrays of either slanted or herringbone grooves were fabricated in poly(dimethylsiloxane) (PDMS) and compared to channels containing no grooves. Mixing in channels with slanted grooves was characterized using a fluorescence method as a function of distance and at different flow rates, and compared to the mixing behavior observed in channels with no grooves. For electrochemical detection, a thin-film Pt electrode was positioned at the end of the fluidic channel as an on-chip detector of the reaction product, H2O 2. Glucose determination was performed by rapidly mixing glucose and glucose oxidase (GOx) in solution at a flow rate of 0.5 μL/min and 1.5 μL/min, respectively. A 150 U/mL GOx solution was selected as the optimum concentration of enzyme. In order to investigate the dependence of device response on flow rate, experiments with a premixed solution of glucose and GOx were compared to experiments in which glucose and GOx were reacted on-chip. Calibration curves for glucose (0-20 mM, in the clinical range of interest) were obtained in channels with and without grooves, using amperometric detection and a 150 U/mL GOx solution for in-chip reaction. © 2010 American Chemical Society.
Subject
Biology
Biomedical Research
Amperometric detection
Calibration curves
Chaotic mixing
Continuous glucose monitoring
Device response
ELectrochemical detection
Fluidic channels
Fluorescence method
Glucose determination
In-channels
In-chip
Micro-mixing
Micro-reactor
Mixing behaviors
On chips
Optimum concentration
Polydimethylsiloxane PDMS
Premixed
Pt electrode
Sensing systems
Chemical detection
Glucose
Glucose sensors
Mixing
Monitoring
Platinum
Sensors
Glucose oxidase
To reference this document use:
http://resolver.tudelft.nl/uuid:616dc712-1ffb-4508-82c5-22515afdfcb6
TNO identifier
409281
ISSN
0003-2700
Source
Analytical Chemistry, 82 (16), 6756-6763
Document type
article