Title
The complete karyotype of Aspergillus niger: The use of introduced electrophoretic mobility variation of chromosomes for gene assignment studies
Author
Verdoes, J.C.
Calil, M.R.
Punt, P.J.
Debets, A.J.M.
Swart, K.
Stouthamer, A.H.
van den Hondel, C.A.M.J.J.
Publication year
1994
Abstract
A method is described for unambiguous assignment of cloned genes to Aspergillus niger chromosomes by CHEF gel electrophoresis and Southern ana lysis. All of the eight linkage groups (LGs), with the exception of LG VII, have previously been assigned to specific chromosomal bands in the electrophoretic karyotype of A. niger. Using a LG VII-specific probe (nicB gene of A. niger) we have shown that LG VII corresponds to a chromosome of about 4.1 Mb. Furthermore, genetic localization of three unassigned genes (glaA, aglA and pepA) in strains in which these genes had been replaced by a selectable marker gene led to a revised karyotype for the chromosomes corresponding to LGs VIII and VI. The revised electrophoretic karyotype reveals only 5 distinct bands. The presence of three pairs of equally sized chromosomes precluded assignment of genes to one specific chromosome in the wild-type strain. However, unambiguous chromosome assignment of cloned genes using CHEF-Southern analysis was demonstrated using a set of A. niger strains with introduced chromosomal size variation. The availability of these tester strains obviates the need to isolate or construct mutant strains for the purpose of chromosome assignment.
Subject
Contour-clamped homogeneous electric field (CHEF) analysis Filamentous fungi
Gene replacement
Linkage group analysis
Aspergillus niger
Gel electrophoresis
Gene assignment
Gene location
Genetic linkage
Karyotype
Nonhuman
Priority journal
Aspergillus niger
Chromosome Mapping
Chromosomes, Fungal
Electrophoresis, Agar Gel
Karyotyping
Linkage (Genetics)
Support, Non-U.S. Gov't
Aspergillus niger
Fungi
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DOI
https://doi.org/10.1007/bf00280189
TNO identifier
67078
Source
Molecular and General Genetics, 244, 75-80
Document type
article