Print Email Facebook Twitter High-performance liquid chromatographic determination of urinary free catecholamines with electrochemical detection after prepurification on immobilized boric acid Title High-performance liquid chromatographic determination of urinary free catecholamines with electrochemical detection after prepurification on immobilized boric acid Author Speek, A.J. Odink, J. Schrijver, J. Schreurs, W.H.P. Centraal Instituut voor Voedingsonderzoek TNO Publication year 1983 Abstract A reliable high-performance liquid chromatographic method is presented for the determination of the urinary free catecholamines noradrenaline, adrenaline and dopamine. Urine is purified on a column of immobilized boric acid. Catecholamines are separated by ion-pair reversed phase high-performance liquid chromatography and detected electrochemically. The method is suited for routine analysis. It allows the determination of urinary free catecholamines in concentrations as low as 1 ??g/l for noradrenaline and adrenaline and 5 ??g/l for dopamine. A single analysis can be completed within 1 h. Routine analyses can be carried out in a series of 40 samples within 2 days. The within-assay and between-assay coefficients of variation of the analyses in urine were both 2.9% for noradrenaline, both 5.0% for adrenaline, and 1.9 and 2.1% for dopamine. The chromatographic properties of the immobilized boric acid were investigated. In particular, the elution pattern of a series of catecholamine metabolites and analogues was determined. Under the conditions used, only basic compounds containing both a vicinal hydroxyl configuration and a primary or secondary amino group adsorb and elute together with the free catecholamines. Chemicals/CAS: adrenalin, 51-43-4, 55-31-2, 6912-68-1; alpha methyldopamine, 555-64-6; dopamine, 51-61-6, 62-31-7; isoprenaline, 299-95-6, 51-30-9, 6700-39-6, 7683-59-2; noradrenalin, 1407-84-7, 51-41-2; boric acid, 11113-50-1; Boric Acids; Catecholamines Subject AdrenalinAlpha methyldopamineCatecholamineDopamineIsoprenalineNoradrenalinDrug analysisDrug determinationDrug urine levelLiquid chromatographyNonhumanTheoretical studyWineBoric AcidsCatecholaminesChromatography, High Pressure LiquidElectrochemistryHuman To reference this document use: http://resolver.tudelft.nl/uuid:5f6664f3-4d54-41e3-84e4-1aa14a3976a8 DOI https://doi.org/10.1016/0009-8981(83)90060-8 TNO identifier 229369 ISSN 0009-8981 Source Clinica Chimica Acta, 128 (1), 103-113 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.