Title
Determining the long-term effect of antibiotic administration on the human normal intestinal microbiota using culture and pyrosequencing methods
Author
Rashid, M.U.
Zaura, E.
Buijs, M.J.
Keijser, B.J.F.
Crielaard, W.
Nord, C.E.
Weintraub, A.
Publication year
2015
Abstract
The purpose of the study was to assess the effect of ciprofloxacin (500 mg twice daily for 10 days) or clindamycin (150 mg 4 times daily for 10 days) on the fecal microbiota of healthy humans for a period of 1 year as compared to placebo. Two different methods, culture and microbiome analysis, were used. Fecal samples were collected for analyses at 6 time-points. The interval needed for the normal microbiota to be normalized after ciprofloxacin or clindamycin treatment differed for various bacterial species. It took 1-12 months to normalize the human microbiota after antibiotic administration, with the most pronounced effect on day 11. Exposure to ciprofloxacin or clindamycin had a strong effect on the diversity of the microbiome, and changes in microbial composition were observed until the 12th month, with the most pronounced microbial shift at month 1. No Clostridium difficile colonization or C. difficile infections were reported. Based on the pyrosequencing results, it appears that clindamycin has more impact than ciprofloxacin on the intestinal microbiota. © 2015 The Author 2015. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved.
Subject
Life
MSB - Microbiology and Systems Biology
ELSS - Earth, Life and Social Sciences
Biomedical Innovation
Biology
Healthy Living
Antibiotics
Culture
Intestinal microbiota
Pyrosequencing
To reference this document use:
http://resolver.tudelft.nl/uuid:5f541203-86eb-462c-a696-8c4e6c30b25b
DOI
https://doi.org/10.1093/cid/civ137
TNO identifier
525571
ISSN
1058-4838
Source
Clinical Infectious Diseases, 60, S77-S84
Document type
article