Title
Biochemistry and measurement of fibrinogen
Author
Nieuwenhuizen, W.
TNO Preventie en Gezondheid
Publication year
1995
Abstract
Fibrinogen is a large heterogeneous family of closely related molecules consisting of three pairs of non-identical polypeptide chains: two Aα-, two Bβ- and two γ-chains, held together by disulphide bridges. The heterogeneity of fibrinogen is due to heterogeneities in all three chains. Four main types of assay are used to determine fibrinogen:clotting rate (Clauss), clottable protein, precipitation and immunological assays. Heterogenities may differ from person to person and may affect the apparent fibrinogen concentrations in different assays. A further complicating factor was, until recently, the lack of an international fibrinogen standard. The ratio of Clauss:enzyme immunoassay (EIA) for high + low molecular weight fibrinogen decreases during therapy for acute myocardial infarction and increases again after thrombolytic therapy to above normal values. Furthermore, high molecular weight fibrinogen tends to clot more easily than low molecular weight fibrinogen. This suggests that high molecular weight fibrinogen might be associated with increased thrombolytic risk. Fibrinogen assessed by a functional assay (Clauss) alone is strongly associated with ischaemic heart disease. Although not proven, it is conceivable that a fibrinogen with a Clauss:EIA ratio of > 1 has an even stronger association in epidemiological studies. Chemicals/CAS: disulfide, 16734-12-6; fibrinogen, 9001-32-5; Fibrinogen, 9001-32-5
Subject
Biology
Fibrinogen
Functionality of fibrinogen
Measurement
Molecular heterogeneity
Polymerization
disulfide
fibrinogen
acute heart infarction
blood clotting
disulfide bond
enzyme immunoassay
fibrinolytic therapy
ischemic heart disease
molecular weight
priority journal
protein polymorphism
review
risk
thrombosis
Cardiovascular Diseases
Coronary Disease
Fibrinogen
Human
Immunoassay
Molecular Weight
Precipitation
Reference Values
Whole Blood Coagulation Time
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TNO identifier
232844
ISSN
0195-668X
Source
European Heart Journal, 16 (SUPPL. A), 6-10
Document type
article