Print Email Facebook Twitter Gas chromatographic determination of deoxynivalenol in cereals Title Gas chromatographic determination of deoxynivalenol in cereals Author Mulders, Ed.J. van Impelen-Peek, H.A.M. Centraal Instituut voor Voedingsonderzoek TNO Publication year 1986 Abstract Samples of barley, wheat and maize were analysed for deoxynivalenol (DON). Samples were extracted with aqueous chloroform-ethanol. The extract was purified by column chromatography on silica gel. DON was derivatised with heptafluorobutyric (HFB) acid anhydride. The glassware was silylated to prevent deterioration of the HFB derivative, which stabilised the samples for at least 11 weeks. Quantitation was done by capillary gas chromatography using electron-capture detection. Confirmation was obtained by gas chromatography-mass spectrometry. The limit of determination was 0.02 mg/kg. Recoveries of DON averaged 99% with a coefficient of variation of 10.8%. ?? 1986 Springer-Verlag. Chemicals/CAS: vomitoxin, 51481-10-8; deoxynivalenol, 51481-10-8; Sesquiterpenes; Trichothecenes Subject Trichothecene derivativeVomitoxinAnimalCerealFood contaminationFood controlGas chromatographyAnimalCerealsChromatography, GasFood ContaminationFood MicrobiologyHumanSesquiterpenesTrichothecenes To reference this document use: http://resolver.tudelft.nl/uuid:5c145ede-1904-4b90-9024-0197ea40c481 DOI https://doi.org/10.1007/bf01140549 TNO identifier 230230 ISSN 0044-3026 Source Zeitschrift fur Lebensmittel-Untersuchung und -Forschung, 183 (6), 406-409 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.