Print Email Facebook Twitter Functions of the CCCH type zinc finger protein OsGZF1 in regulation of the seed storage protein GluB-1 from rice Title Functions of the CCCH type zinc finger protein OsGZF1 in regulation of the seed storage protein GluB-1 from rice Author Chen, Y. Sun, A. Wang, M. Zhu, Z. Ouwerkerk, P.B.F. Centraal Instituut voor Voedingsonderzoek TNO Publication year 2014 Abstract Glutelins are the most abundant storage proteins in rice grain and can make up to 80 % of total protein content. The promoter region of GluB-1, one of the glutelin genes in rice, has been intensively used as a model to understand regulation of seed-storage protein accumulation. In this study, we describe a zinc finger gene of the Cys3His1 (CCCH or C3H) class, named OsGZF1, which was identified in a yeast one-hybrid screening using the core promoter region of GluB-1 as bait and cDNA expression libraries prepared from developing rice panicles and grains as prey. The OsGZF1 protein binds specifically to the bait sequence in yeast and this interaction was confirmed in vitro. OsGZF1 is predominantly expressed in a confined domain surrounding the scutellum of the developing embryo and is localised in the nucleus. Transient expression experiments demonstrated that OsGZF1 can down-regulate a GluB-1-GUS (β-glucuronidase) reporter and OsGZF1 was also able to significantly reduce activation conferred by RISBZ1 which is a known strong GluB-1 activator. Furthermore, down-regulation of OsGZF1 by an RNAi approach increased grain nitrogen concentration. We propose that OsGZF1 has a function in regulating the GluB-1 promoter and controls accumulation of glutelins during grain development. © 2013 Springer Science+Business Media Dordrecht. Subject LifeMSB - Microbiology and Systems BiologyELSS - Earth, Life and Social SciencesHealthy for LifeBiologyHealthy Living Healthy LivingCCCHGluB-1GlutelinRepressorRiceZinc fingerDNA binding proteinGluB 1 protein, OryzaGluB-1 protein, OryzaGlutenNitrogenVegetable proteinZinc finger proteinBinding siteCytologyDNA sequenceDown regulationGene expression regulationGene libraryGeneticsMetabolismMolecular geneticsNucleotide sequencePhysiologyPlant seedPromoter regionReporter geneRiceRNA interferenceTransgenic plantTwo hybrid systemBase SequenceBinding SitesDNA-Binding ProteinsDown-RegulationGene Expression Regulation, PlantGene LibraryGenes, ReporterGlutensMolecular Sequence DataNitrogenOryza sativaPlant ProteinsPlants, Genetically ModifiedPromoter Regions, GeneticRNA InterferenceSeedsSequence Analysis, DNATwo-Hybrid System TechniquesZinc Fingers To reference this document use: http://resolver.tudelft.nl/uuid:5c056948-94cc-426a-9136-d58acb349ab8 DOI https://doi.org/10.1007/s11103-013-0158-5 TNO identifier 507114 ISSN 0167-4412 Source Plant Molecular Biology, 84 (6), 621-634 Bibliographical note Molecular Sequence Numbers: GENBANK: AK108315, KF289071; Chemicals/CAS: gluten, 8002-80-0; nitrogen, 7727-37-9 Funding Details: KNAW, Royal Netherlands Academy of Arts and Sciences Document type article Files To receive the publication files, please send an e-mail request to TNO Library.