Title
Establishing a campylobacter-free pig population through a top-down approach
Author
Weijtens, M.J.B.M.
Urlings, H.A.P.
van der Plas, J.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
2000
Abstract
Fattening pigs are often infected with campylobacter. To eliminate campylobacter from the pig population, a top-down approach, involving the breeding and reproduction farms, seems appropriate. In order to investigate the effectiveness of a top-down approach, sows' faeces from the following farms were analysed for the presence of campylobacter: one specific pathogen free (SPF) farm, three top-breeding farms with no connection with SPF breeding, and a breeding farm repopulated with SPF sows after a period of vacancy (farm 5). The faeces samples from the SPF farm were free from campylobacter. The three top-breeding farms provided faeces samples which were 98% positive for campylobacter. However, only 22% of the faeces samples from farm 5 were positive for campylobacter. In a period of 20 months, the percentage of sows infected with campylobacter on farm 5 did not significantly increase. Genetic typing with ERIC-PCR and RFLP of campylobacter isolates from one of the top-breeding farms and from farm 5 showed a high diversity of campylobacter types. The results suggest that a campylobacter-free pig population can be established in breeding farms by combining a top-down approach (campylobacter-free top-breeding farms) with a strict regime of hygiene management.
Subject
Animal experiment
Bacterium identification
Bacterium isolate
Breeding
Campylobacter
Controlled study
Feces analysis
Genetic screening
Gram negative infection
Hygiene
Infection control
Nonhuman
Polymerase chain reaction
Restriction fragment length polymorphism
Swine
Animal Husbandry
Animals
Breeding
Campylobacter
DNA, Bacterial
Feces
Genotype
Polymorphism, Restriction Fragment Length
Random Amplified Polymorphic DNA Technique
Specific Pathogen-Free Organisms
Swine
Variation (Genetics)
Animalia
Bacteria (microorganisms)
Campylobacter
Negibacteria
Sus scrofa
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TNO identifier
71942
ISSN
0266-8254
Source
Letters in Applied Microbiology, 30 (6), 479-484
Document type
article