Title
Alveolar epithelial cells (A549) exposed at the air-liquid interface to diesel exhaust: First study in TNO's powertrain test center
Author
Kooter, I.M.
Alblas, M.J.
Jedynska, A.D.
Steenhof, M.
Houtzager, M.M.G.
van Ras, M.G.
Publication year
2013
Abstract
Air–liquid interface (ALI) exposures enable in vitro testing ofmixtures of gases and particles such as diesel exhaust (DE). The main objective of this study was to investigate the feasibility of exposing human lung epithelial cells at the ALI to complete DE generated by a heavy-duty truck in the state-of-the-art TNO powertrain test center. A549 cells were exposed at the air–liquid interface to DE generated by a heavy-duty Euro III truck for 1.5 h. The truck was tested at a speed of 70 km/h-1 to stimulate free-flowing traffic on a motorway. Twenty-four hours after exposure, cells were analyzed for markers of oxidative stress (GSH and HO-1), cytotoxicity (LDH) and Alamar Blue assay) and inflammation (IL-8). DE exposure resulted in an increased oxidative stress response (significantly increased HO-1 levels and significantly reduced GSH/GSSH ratio), and a decreased cell viability (significantly decreased Alamar Blue levels and slightly increased LDH levels). However, the pro-inflammatory response seemed to decrease (decrease in IL-8). The results presented here demonstrate that we are able to successfully expose A549 cells at ALI to complete DE generated by a heavy-duty truck in TNO’s powertrain test center and show oxidative stress and cytotoxicity responses due to DE exposure
Subject
Earth & Environment Life Fluid Mechanics Chemistry & Energetics Life
AEC - Applied Environmental Chemistry CBRN - CBRN Protection PT - Power Trains RAPID - Risk Analysis for Products in Development
EELS - Earth, Environmental and Life Sciences TS - Technical Sciences
Urban Development
Emission
Built Environment
To reference this document use:
http://resolver.tudelft.nl/uuid:5963b22e-9f06-4378-9736-a0dca31494bc
TNO identifier
481022
Source
Toxicology in Vitro, 27 (8), 2342-2349
Document type
article