Print Email Facebook Twitter Alveolar epithelial cells (A549) exposed at the air-liquid interface to diesel exhaust: First study in TNO's powertrain test center Title Alveolar epithelial cells (A549) exposed at the air-liquid interface to diesel exhaust: First study in TNO's powertrain test center Author Kooter, I.M. Alblas, M.J. Jedynska, A.D. Steenhof, M. Houtzager, M.M.G. van Ras, M.G. Publication year 2013 Abstract Air–liquid interface (ALI) exposures enable in vitro testing ofmixtures of gases and particles such as diesel exhaust (DE). The main objective of this study was to investigate the feasibility of exposing human lung epithelial cells at the ALI to complete DE generated by a heavy-duty truck in the state-of-the-art TNO powertrain test center. A549 cells were exposed at the air–liquid interface to DE generated by a heavy-duty Euro III truck for 1.5 h. The truck was tested at a speed of 70 km/h-1 to stimulate free-flowing traffic on a motorway. Twenty-four hours after exposure, cells were analyzed for markers of oxidative stress (GSH and HO-1), cytotoxicity (LDH) and Alamar Blue assay) and inflammation (IL-8). DE exposure resulted in an increased oxidative stress response (significantly increased HO-1 levels and significantly reduced GSH/GSSH ratio), and a decreased cell viability (significantly decreased Alamar Blue levels and slightly increased LDH levels). However, the pro-inflammatory response seemed to decrease (decrease in IL-8). The results presented here demonstrate that we are able to successfully expose A549 cells at ALI to complete DE generated by a heavy-duty truck in TNO’s powertrain test center and show oxidative stress and cytotoxicity responses due to DE exposure Subject Earth & Environment Life Fluid Mechanics Chemistry & Energetics LifeAEC - Applied Environmental Chemistry CBRN - CBRN Protection PT - Power Trains RAPID - Risk Analysis for Products in DevelopmentEELS - Earth, Environmental and Life Sciences TS - Technical SciencesUrban DevelopmentEmissionBuilt Environment To reference this document use: http://resolver.tudelft.nl/uuid:5963b22e-9f06-4378-9736-a0dca31494bc TNO identifier 481022 Source Toxicology in Vitro, 27 (8), 2342-2349 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.