Title
Eradication of Helicobacter pylori infection favourably affects altered gastric mucosal MMP-9 levels
Author
Kubben, F.J.G.M.
Sier, C.F.M.
Schram, M.
Witte, T.A.M.C.
Veenendaal, R.A.
van Duijn, W.
Verheijen, J.H.
Hanemaaijer, R.
Lamers, C.B.H.W.
Verspaget, H.W.
TNO Kwaliteit van Leven
Publication year
2007
Abstract
Background: Helicobacter pylori gastritis is recognized as an important pathogenetic factor in peptic ulcer disease and gastric carcinogenesis, and is accompanied by strongly enhanced gastric mucosal matrix metalloproteinase-9 (MMP-9) levels. Aim: This study was performed to investigate whether H. pylori-affected gastric mucosal MMP-2 and MMP-9 levels are reversible by successful treatment of the infection. Patients and methods: Fifty-eight patients with H. pylori-associated gastritis were treated with a combination regimen of acid inhibitory therapy and antibiotics for 14 days. The levels and isoforms of MMP-2 and MMP-9 were measured by semiquantitative gelatin-zymography, bioactivity assay and enzyme-linked immunosorbent assay in gastric mucosal biopsy homogenates. Results: Latent, active, and total MMP-9 levels decreased consistently and significantly by successful H. pylori eradication, in antrum as well as corpus mucosa, compared with those prior to treatment, irrespective of the therapy regimen used. The elevated levels remained unchanged, however, when treatment failed. MMP-2 levels did not show major alterations after H. pylori therapy. Conclusion: Elevated MMP-9 levels in H. pylori-infected gastric mucosa are reversible by eradication of the infection. No major changes in mucosal MMP-2 levels were observed by H. pylori eradication. © 2007 The Authors.
Subject
Biomedical Research
Bioactivity assay (BIA)
ELISA
Gastritis
Helicobacter pylori
Matrix metalloproteinases
MMP-2 (gelatinase A)
MMP-9 (gelatinase B)
Zymography
antibiotic agent
clarithromycin
gelatin
gelatinase A
gelatinase B
metronidazole
omeprazole
proton pump inhibitor
ranitidine
adult
aged
article
controlled study
drug activity
enzyme analysis
enzyme linked immunosorbent assay
eradication therapy
female
Helicobacter infection
Helicobacter pylori
human
major clinical study
male
priority journal
quantitative analysis
statistical significance
stomach mucosa
zymography
Adult
Aged
Anti-Infective Agents
Anti-Ulcer Agents
Biopsy
Clarithromycin
Drug Therapy, Combination
Endoscopy, Gastrointestinal
Enzyme-Linked Immunosorbent Assay
Female
Gastric Mucosa
Helicobacter Infections
Helicobacter pylori
Humans
Male
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Metronidazole
Middle Aged
Omeprazole
Ranitidine
Treatment Outcome
Helicobacter pylori
To reference this document use:
http://resolver.tudelft.nl/uuid:580eb497-0f38-4b3d-87c6-4c86cf5ef4d2
DOI
https://doi.org/10.1111/j.1523-5378.2007.00527.x
TNO identifier
240229
ISSN
1083-4389
Source
Helicobacter, 12 (5), 498-504
Document type
article