Title
Cytotoxicity of dental composite components and mercury compounds in lung cells
Author
Reichl, F.X.
Walther, U.I.
Durner, J.
Kehe, K.
Hickel, R.
Kunzelmann, K.H.
Spahl, W.
Hume, W.R.
Benschop, H.P.
Forth, W.
Publication year
2001
Abstract
Objective: The effect of dental composite components triethyleneglycoldimethacrylate (TEGDMA) and hydroxyethylmethacrylate (HEMA), as well as mercuric chloride (HgCl2) and methylmercury chloride (MeHgCl) was investigated on the release of lactatedehydrogenase (LDH) from alveolar epithelial lung cell lines in vitro. Methods: The confluent cell layers from the A549 (human, malignant) and the L2 cells (rat) were incubated with various concentrations of HEMA, TEGDMA, MeHgCl and HgCl2 at 37°C in 2% (v/v) CO2 atmosphere for 8 h. In further experiments the L2 cells were incubated with the same compounds for 6-48 h. LDH release was measured and the values were expressed as percentage of the LDH content. The values were plotted on a concentration log-scale and the substance concentration at the maximum slope was assessed as effective concentration (EC50). Results: A significant (p < 0.05) increase in the LDH release was found in the L2 cells after 8-h incubation with HEMA (4 mmol/1), TEGDMA (2 mmol/l), MeHgCl (0.01 mmol/l) and HgCl2 (0.015 mmol/l), and in A549 cells with HEMA (14 mmol/l), TEGDMA (15 mmol/l), MeHgCl (0.15 mmol/l) and HgCl2 (0.05 mmol/l), compared to controls. The EC50 values from compounds in the L2 cells are shown in the following table (mean; sem in parentheses; n = 3-6; #n = 1): Time (h) 6 12 24 36 48 HEMA 5.46 (1.61) 4.66 (1.97) 3.68 (1.15) 3.22 (1.38) 0.59 (0.17) TEGDMA 3.37 (1.65) 1.30 (0.39) 1.47 (0.33) 1.58 (0.28) 0.42 (0.10) MeHgCl 0.018 (0.004) 0.007 (0.001) 0.009 (0.000) 0.009 (0.000) 0.003 (0.002) HgCl2 0.016 (0.000) 0.014 (0.000) 0.009 (0.003) 0.008 (0.004) 0.007# Significance: The toxic effect of HgCl2 and MeHgCl from the L2 cells was about 100-700-fold higher than of the dental composite components. A significant (p < 0.05) time dependent increase of toxicity was observed with TEGDMA, HEMA and MeHgCl. © 2001 Academy of Dental Materials. Published by Elsevier Science Ltd. All rights reserved.
Subject
Cytotoxicity
Dental material
HEMA
Lactatedehydrogenase
Lung cells
Mercuric chloride
Methylmercury chloride
TEGDMA
2 hydroxyethyl methacrylate
Biomaterial
Biomedical and dental materials
Lactate dehydrogenase
Macrogol derivative
Mercury derivative
Methacrylic acid derivative
Methylmercuric chloride
Methylmercury derivative
Polymethacrylic acid derivative
Resin
Topical antiinfective agent
Triethylene glycol dimethacrylate
Animal
Cell culture
Cell line
Cytology
Drug effect
Epithelium cell
Human
Lung
Lung alveolus
Lung tumor
Pathology
Rat
Statistical model
Statistics
Temperature
Time
Anti-infective agents, local
Biocompatible materials
Cell line
Composite resins
Dental materials
Epithelial cells
Linear models
Mercury Compounds
Methacrylates
Methylmercury compounds
Polyethylene glycols
Polymethacrylic acids
Pulmonary alveoli
Time factors
Tumor cells, Cultured
Hydroxyethyl methacrylate, 868-77-9
L-Lactate Dehydrogenase, EC 1.1.1.27
Mercuric chloride, 7487-94-7
Methylmercuric chloride, 115-09-3
Triethylene glycol dimethacrylate, 109-16-0
To reference this document use:
http://resolver.tudelft.nl/uuid:56e8e7ec-bd40-49db-899e-91da692ff2dc
DOI
https://doi.org/10.1016/s0109-5641(00)00029-4
TNO identifier
235994
Source
Dental Materials, 17 (17), 95-101
Document type
article