Title
An expression system based on the promoter region of the Aspergillus awamori 1,4-bèta-endoxylanase A gene
Author
Gouka, R.J.
Hessing, J.G.M.
Punt, P.J.
Stam, H.
Musters, W.
van den Hondel, C.A.M.J.J.
TNO Voeding
Publication year
1996
Abstract
A new, highly inducible fungal promoter derived from the Aspergillus awamori 1,4-β-endoxylanase A (exlA) gene is described. Induction analysis, carried out with the wild-type strain in shake flasks, showed that exlA expression is regulated at the transcriptional level. Using a β-glucuronidase (uidA) reporter strategy, D-xylose was shown to be an efficient inducer of the exlA promoter, whereas sucrose or maltodextrin were not. Upon D-xylose induction, the exlA promoter was threefold more efficient than the frequently used A. niger glucoamylase (glaA) promoter under maltodextrin induction. Detailed induction analyses demonstrated that induction was-dependent on the presence of D-xylose in the medium. Carbon-source-limited chemostat cultures with the uidA reporter strain showed that D-xylose was also a very good inducer in a fermenter, even in the presence of sucrose.
Subject
Nutrition
Enzyme
Glycosidase
Microbial enzyme
Controlled study
Gene expression regulation
Molecular cloning
Nonhuman
Promoter region
Aspergillus
Endo-1,4-beta Xylanases
Enzyme Induction
Gene Expression Regulation, Fungal
Genes, Reporter
Genetic Vectors
Glucan 1,4-alpha-Glucosidase
Glucuronidase
Promoter Regions (Genetics)
RNA, Fungal
RNA, Messenger
Transcription, Genetic
Xylose
Xylosidases
Aspergillus awamori
Aspergillus niger
Trixis
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DOI
https://doi.org/10.1007/s002530050779
TNO identifier
68889
Source
Appied Microbiology and Biotechnology, 46, 28-35
Document type
article