Title
Effects of innervation by ciliary ganglia on developing muscle in vitro
Author
Slaaf, D.W.
Hooisma, J.
Meeter, E.
Stevens, W.F.
Medisch Biologisch Laboratorium TNO
Publication year
1979
Abstract
Electrophysiological and pharmacological properties of neuromuscular junctions formed in tissue culture between chick ciliary ganglia and chick skeletal muscle cells have been studied. Functional neuromuscular junctions are formed already within 24h. No functional acetylcholine (ACh) esterase is present at these endplates. The neurites conduct action potentials to the neuromuscular junctions, where EPPs are generated. Tetrodotoxin (TTX) blocks this nerve conduction but in the presence of TTX MEPP-like potentials remain whose amplitudes are lowered when the Mg2+/Ca2+ ratio in the medium is raised. It is speculated that these large TTX-resistant potentials are multiquantal. The relatively high resting membrane potential in non innervated muscle fibers was not changed by innervation. ACh-sensitivity was determined by iontophoretical application of ACh to the myotubes. Non-innervated myotubes exhibited an evenly distributed ACh-sensitivity. Local differences in ACh-sensitivity were always gradual and never exceeded a factor of 3. Innervation did not alter the overall ACh-sensitivity, but on functionally innervated muscle cells loci hypersensitive to ACh were found. Hypersensitivity was located within sharply defined areas. Apparently the para-sympathetic neurons of the ciliary ganglion are able to form functional neuromuscular junctions with skeletal muscle cells in tissue culture and to induce the formation of regions of high ACh-sensitivity. Chemicals/CAS: acetylcholine, 51-84-3, 60-31-1, 66-23-9; acetylcholinesterase, 9000-81-1; tetrodotoxin, 4368-28-9, 4664-41-9; Atropine, 51-55-8; Physostigmine, 57-47-6; Tetrodotoxin, 4368-28-9; Tubocurarine, 57-95-4
Subject
Biology
Acetylcholine
Acetylcholinesterase
Tetrodotoxin
Animal experiment
Chicken
Ciliary ganglion
Endplate potential
In vitro study
Muscle
Neuromuscular synapse
Tissue culture
Animal
Atropine
Cells, Cultured
Chick Embryo
Electric Stimulation
Ganglia, Parasympathetic
Membrane Potentials
Motor Endplate
Muscles
Physostigmine
Tetrodotoxin
Tubocurarine
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DOI
https://doi.org/10.1016/0006-8993(79)90516-x
TNO identifier
228640
ISSN
0006-8993
Source
Brain Research, 175 (1), 87-107
Document type
article