Title
Functions of OsDof25 in regulation of OsC4PPDK
Author
Zhang, Y.
Verhoeff, N.I.
Chen, Z.
Chen, S.
Wang, M.
Zhu, Z.
Ouwerkerk, P.B.F.
Publication year
2015
Abstract
Relative little is known about the functions of the so-called Dof zinc factors in plants. Here we report on the analysis of OsDof25 and show a function in regulation of the important C4 photosynthesis gene, OsC4PPDK in rice. Over-expression of OsDof25 enhanced the expression of OsC4PPDK in transient expression experiments by binding in a specific way to a conserved Dof binding site which was confirmed by yeast and in vitro binding studies. Expression studies using promoter GUS plants as well as qPCR experiments showed that OsDof25 expressed in different tissues including both photosynthetic and non-photosynthetic organs and that expression of OsDof25 was partially overlapping with the OsC4PPDK gene. Conclusive evidence for a role of OsDof25 in regulation of C4PPDK came from loss-of-function and gain-of-function experiments with transgenic rice, which showed that down-regulation or over-expression of OsDof25 correlated with OsC4PPDK expression and that OsDof25 has functions as transcriptional activator. © 2015, The Author(s).
Subject
Staf
PO - Projectorganisatie
ELSS - Earth, Life and Social Sciences
Biomedical Innovation
Biology
Healthy Living
Dof
OsC4PPDK
Rice
Transcription factor
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DOI
https://doi.org/10.1007/s11103-015-0357-3
TNO identifier
528435
ISSN
0167-4412
Source
Plant Molecular Biology, 89 (3), 229-242
Document type
article