Print Email Facebook Twitter No effects of PCSK9-inhibitor treatment on spatial learning, locomotor activity, and novel object recognition in mice Title No effects of PCSK9-inhibitor treatment on spatial learning, locomotor activity, and novel object recognition in mice Author Schlunk, F. Fisher, P. Princen, H.M.G. Rex, A. Prinz, V. Foddis, M. Lütjohann, D. Laufs, U. Endres, M. Publication year 2021 Abstract Monoclonal anti-proprotein convertase subtilisin/kexin type 9 (PSCK9) neutralizing antibodies effectively lower plasma cholesterol levels and decrease cardiovascular events but also raised some concern that cognitive function could worsen as a side effect. Here, we performed experiments in mice to characterize the effect of anti-PCSK9 antibodies on behavior and cognitive function in detail. APOE*3Leiden.CETP mice and B6129SF1/J wildtype mice were fed a Western type diet and treated with the fully human anti-PCSK9 antibody CmAb1 (PL-45134; 10mg*kg-1 s.c.) or vehicle for 6 weeks. Locomotor activity, anxiety levels, recognition memory, and spatial learning were investigated using the open field, novel object recognition test, and Morris water maze, respectively. Serum cholesterol levels in APOE*3Leiden.CETP mice after treatment with anti-PCSK9 antibody were significantly lower compared to controls whereas cholesterol levels in B6129SF1/J wildtype mice remained unchanged at low levels. No apparent differences were found regarding locomotor activity, anxiety, recognition memory, and spatial learning between animals treated with anti-PCSK9 antibody or vehicle in APOE*3Leiden.CETP and B6129SF1/J wildtype mice. In this study, we found no evidence that treatment with anti-PCSK9 antibodies lead to differences in behavior or changes of cognition in mice. Subject LifeMHR - Metabolic Health ResearchELSS - Earth, Life and Social SciencesBiomedical InnovationHealthy LivingCognititionAnimal researchPCSK9-inhibitorsCholesterolBehaviour To reference this document use: http://resolver.tudelft.nl/uuid:54a064ac-62fe-44ec-a6b6-1b2152d34cfa DOI https://doi.org/10.1016/j.bbr.2020.112875 TNO identifier 880213 Source Behavioral Brain Research, 396 Article number 112875 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.