Title
Gender-dependent effects of high-fat lard diet on cardiac function in C57Bl/6J mice
Author
Louwe, M.C.
van der Hoorn, J.W.A.
van den Berg, S.A.A.
Wouter Jukema, J.
Romijn, J.A.
van Dijk, K.W.
Rensen, P.C.N.
Smit, J.W.A.
Steendijk, P.
Publication year
2012
Abstract
Increased availability of fatty acids released from insulin-resistant adipose tissue may lead to excess fatty acid uptake in nonadipose organs, including the heart. Accumulation of toxic fatty acid intermediates may affect cardiac function. Our aim was to identify to which extent high-fat diet feeding leads to alterations in cardiac function and whether this depends on gender and (or) duration of high-fat diet feeding. Male and female C57Bl/6J mice (n = 8 per group) of 12 to 16 weeks old were fed a low-fat (10% energy) or high-fat (45% energy) lard diet for 6 or 12 weeks. Plasma lipid levels, echocardiography, and left ventricular pressure-volume relationships were obtained at 2, 1, and 0 weeks before termination, respectively. In both male and female mice, the high-fat diet increased body weight and plasma lipid content. At 10 weeks, significant increases were observed for plasma total cholesterol (males: +44%; females: +86%), phospholipids (+16% and +34%), and triglycerides (+27% and +53%) (all p < 0.001). In male mice, but not in female mice, the high-fat diet significantly affected cardiac function at 12 weeks with increased end-systolic volume (25.4 ± 6.2 vs. 17.0 ± 6.7 μL, p <0.05), increased end-systolic pressure (72.1 ± 6.9 vs. 63.6 ± 6.9 mm Hg, p < 0.01), and decreased ejection fraction (61.2% ± 4.5% vs. 68.1% ± 3.7%, p < 0.01), indicating reduced systolic function. Multiple linear regression analysis indicated a significant diet-gender interaction for end-systolic volume and ejection fraction. In conclusion, high-fat diet feeding increased body weight and plasma lipid levels in male and in female mice, but resulted in impairment of cardiac function only in males.
Subject
Life
MHR - Metabolic Health Research
EELS - Earth, Environmental and Life Sciences
Healthy for Life
Biology
Healthy Living
Gender
Heart function
High-fat diet
Obesity
Pressure-volume loops
To reference this document use:
http://resolver.tudelft.nl/uuid:50dc16e4-4e21-44a3-8da1-ca422a58fcfa
DOI
https://doi.org/10.1139/h11-153
TNO identifier
452788
ISSN
1715-5312
Source
Applied Physiology, Nutrition and Metabolism, 37 (2), 214-224
Document type
article