Print Email Facebook Twitter Removal of UV-induced DNA lesions in mouse epidermis soon after irradiation Title Removal of UV-induced DNA lesions in mouse epidermis soon after irradiation Author Vink, A.A. Bergen Henegouwen, J.B.A. Nikaido, O. Baan, R.A. Roza, L. Centraal Instituut voor Voedingsonderzoek TNO Publication year 1994 Abstract Induction and removal of cyclobutane thymine dimers and (6-4)photoproducts were studied in epidermal DNA isolated from UV-exposed hairless mice. For the detection of DNA damage, lesion-specific monoclonal antibodies were used in an immunoslotblot assay. Following the exposure of mice to 3.0 kJ m-2 UV-B, substantial removal of both thymine dimers (66%) and (6-4)photoproducts (77%) was observed at 24 h after irradiation. No removal, however, was detected at 4 h after irradiation. In contrast, immunofluorescence data obtained previously showed a rapid initial dimer removal after irradiation with 1.0.kJ m-2 UV-B (A.A. Vink, R.J.W. Berg, F.R. De Gruijl, L. Roza and R.A. Baan, Carcinogenesis l2 (1991) 861-64.) Reinvestigation of the removal of dimers and (6-4)photoproducts shortly after three different W doses showed a rapid decrease of both lesions at 2 h after irradiation with 1.0 kJ m-2. The results obtained after irradiations with 2.0 and 3.0 kJ m-2 UV-B suggest a saturation of repair already at 2.0 kJ m-2. Cyclobutane dimers were found to be removed at a lower rate than (6-4)photoproducts. Chemicals/CAS: cyclobutane, 287-23-0; DNA, 9007-49-2; Pyrimidine Dimers Subject (6-4)PhotoproductsCyclobutane thymine dimersHairless miceImmunoblot assayUV-BCyclobutaneMonoclonal antibodyAnimal experimentAnimal modelControlled studyHela cellHuman cellImmunofluorescenceMouseNonhumanUltraviolet irradiationAnimalDNADNA DamageDNA RepairDose-Response Relationship, RadiationEpidermisFemaleHela CellsHumanImmunoblottingKineticsMiceMice, Inbred HRSPyrimidine DimersSupport, Non-U.S. Gov'tTime FactorsUltraviolet Rays To reference this document use: http://resolver.tudelft.nl/uuid:500ab456-72df-420d-9658-4e31f839b5f9 DOI https://doi.org/10.1016/1011-1344(94)07005-9 TNO identifier 82150 Source Journal of Photochemistry and Photobiology B : Biology, 24, 25-31 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.