Title
Removal of UV-induced DNA lesions in mouse epidermis soon after irradiation
Author
Vink, A.A.
Bergen Henegouwen, J.B.A.
Nikaido, O.
Baan, R.A.
Roza, L.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
1994
Abstract
Induction and removal of cyclobutane thymine dimers and (6-4)photoproducts were studied in epidermal DNA isolated from UV-exposed hairless mice. For the detection of DNA damage, lesion-specific monoclonal antibodies were used in an immunoslotblot assay. Following the exposure of mice to 3.0 kJ m-2 UV-B, substantial removal of both thymine dimers (66%) and (6-4)photoproducts (77%) was observed at 24 h after irradiation. No removal, however, was detected at 4 h after irradiation. In contrast, immunofluorescence data obtained previously showed a rapid initial dimer removal after irradiation with 1.0.kJ m-2 UV-B (A.A. Vink, R.J.W. Berg, F.R. De Gruijl, L. Roza and R.A. Baan, Carcinogenesis l2 (1991) 861-64.) Reinvestigation of the removal of dimers and (6-4)photoproducts shortly after three different W doses showed a rapid decrease of both lesions at 2 h after irradiation with 1.0 kJ m-2. The results obtained after irradiations with 2.0 and 3.0 kJ m-2 UV-B suggest a saturation of repair already at 2.0 kJ m-2. Cyclobutane dimers were found to be removed at a lower rate than (6-4)photoproducts. Chemicals/CAS: cyclobutane, 287-23-0; DNA, 9007-49-2; Pyrimidine Dimers
Subject
(6-4)Photoproducts
Cyclobutane thymine dimers
Hairless mice
Immunoblot assay
UV-B
Cyclobutane
Monoclonal antibody
Animal experiment
Animal model
Controlled study
Hela cell
Human cell
Immunofluorescence
Mouse
Nonhuman
Ultraviolet irradiation
Animal
DNA
DNA Damage
DNA Repair
Dose-Response Relationship, Radiation
Epidermis
Female
Hela Cells
Human
Immunoblotting
Kinetics
Mice
Mice, Inbred HRS
Pyrimidine Dimers
Support, Non-U.S. Gov't
Time Factors
Ultraviolet Rays
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DOI
https://doi.org/10.1016/1011-1344(94)07005-9
TNO identifier
82150
Source
Journal of Photochemistry and Photobiology B : Biology, 24, 25-31
Document type
article