Print Email Facebook Twitter Barrier Ëunction in Reconstructed Epidermis and Its Resembiance to Native Human Skin Title Barrier Ëunction in Reconstructed Epidermis and Its Resembiance to Native Human Skin Author Ponec, M. Gibbs, S. GPilgram, G. BoeIsma, E. Koerten, H. Bouwstra, J. Mommaas, M. TNO Voeding Publication year 2001 Abstract One of the prerequisitesforthe use of human skin equivalents for scientific and screening purposes is that their barrierfunction is similar to that of native skin. Using human epidermis reconstructed en de-epidermized dermis we demonstrated that the formation of the stratum corneum (SC) barrier in vitro proceeds similarly as in vivo as judged from the extensive production of lamellar bodies, their complete extrusion atthe stratum granulosum/SC interface, and the formation of multiple broad lamellar structures in the in tercorneocyte space. The presence of well ordered lipid lamellar phases was confirmed by small-angle X-ray diffraction. Although the long periodicity lamellar phase was present in both the native and the reconstructed epidermis, the short periodicity lamellar phase was present only in native tis sue. In addition, the SC lipids predominantly formed the hexagonal sublattice. Analysis of lipid composition revealed that all SC lipids are synthesized in vitro. Differences in SC lipid organization in reconstructed epidermis may be ascribed to the differences in fatty acid content and profile indicating that further improvement in culture conditions is required for generation of in vitro reconstructed epidermiswith stratum barrier properties of the native tissue. Subject LifeRAPID - Risk Analysis for Products in DevelopmentELSS - Earth, Life and Social SciencesFood and NutritionNutritionHealthy LivingStratum corneumBarrier functionReconstructed epidermisLipid composition To reference this document use: http://resolver.tudelft.nl/uuid:4fd7e040-bb85-4027-a7ac-4ef0fbfb13ec TNO identifier 529934 Source Skin Pharmacology and Applied Skin Physiology, 14 (suppl 1), 63-71 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.