Print Email Facebook Twitter Bifidobacterium breve-HT-29 cell line interaction: Modulation of TNF-a induced gene expression Title Bifidobacterium breve-HT-29 cell line interaction: Modulation of TNF-a induced gene expression Author Boesten, R.J. Schuren, F.H.J. Willemsen, L.E.M. Vriesema, A. Knol, J. de Vos, W.M. Publication year 2011 Abstract To provide insight in the molecular basis for intestinal host-microbe interactions, we determined the genome-wide transcriptional response of human intestinal epithelial cells following exposure to cells of Bifidobacterium breve. To select an appropriate test system reflecting inflammatory conditions, the responsiveness to TNF-a was compared in T84, Caco-2 and HT-29 cells. The highest TNF-a response was observed in HT-29 cells and this cell line was selected for exposure to the B. breve strains M-16V, NR246 and UCC2003. After one hour of bacterial pre-incubation followed by two hours of additional TNF-a stimulation, B. breve M-16V (86%), but to a much lesser extent strains NR246 (50%) or UCC2003 (32%), showed a strain-specific reduction of the HT-29 transcriptional response to the inflammatory treatment. The most important functional groups of genes that were transcriptionally suppressed by the presence of B. breve M-16V, were found to be involved in immune regulation and apoptotic processes. About 54% of the TNF-a induced genes were solely suppressed by the presence of B. breve M-16V. These included apoptosis-related cysteine protease caspase 7 (CASP7), interferon regulatory factor 3 (IRF3), amyloid beta (A4) precursor protein-binding family A member 1 (APBA1), NADPH oxidase (NOX5), and leukemia inhibitory factor receptor (LIFR). The extracellular IL-8 concentration was determined by an immunological assay but did not change significantly, indicating that B. breve M-16V only partially modulates the TNF-a pathway. In conclusion, this study shows that B. breve strains modulate gene expression in HT-29 cells under inflammatory conditions in a strain-specific way. © 2011 Wageningen Academic Publishers. Subject LifeMSB - Microbiology and Systems BiologyEELS - Earth, Environmental and Life SciencesNutritionBifidobacteriaInterleukin 8MicroarrayMicrobe-host interactionTranscriptomics To reference this document use: http://resolver.tudelft.nl/uuid:4f504a6a-a592-4c7c-8909-127cb5265f93 DOI https://doi.org/10.3920/bm2011.0005 TNO identifier 436046 ISSN 1876-2883 Source Beneficial Microbes, 2 (2), 115-128 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.