Title
Synchronization of mammalian cells in vitro by inhibition of the DNA synthesis. II. Population dynamics
Author
Medisch Biologisch Labortorium TNO
Galavazi, G.
Bootsma, D.
Publication year
1966
Abstract
Some aspects of the population dynamics before, during and after the double thymidine treatment synchronization procedure were analysed. The mitotic index in the synchronized populations rose from 0.0 per cent at 1 hr after the end of the second treatment to 20.8 per cent at 9.7 hr, dropped to 0.1 per cent at about 22 hr and increased again to a second peak of about 8 per cent at 31 hr. The percentage of mitoses in the controls was 2.1. The degree of synchronization of the first generation, determined according to Engelberg, was 73 per cent. The percentage of S phase cells fell from 96 per cent at 0-3.5 hr via 50 per cent at 6.5 hr to 1 per cent at 12-15 hr, rose to 72 per cent at 24 hr and decreased again to 7 per cent at 34 hr. The mean length of the G1 and the G2 phases of the synchronized cycle was shorter than in the asynchronous controls, while the duration of mitosis and the S phase was about equal in both cases. Some other characteristics of the synchronized generation cycle are discussed. It was argued that the synchronization in this system probably was a consequence of a reversible alteration of the life cycle of the cells, induced by thymidine. The mean duration of the S phase was greatly increased in the presence of thymidine. Some biochemical implications concerning the blocking and deblocking of the DNA synthesis by the administration and removal of thymidine are discussed. © 1966.
Subject
Antimetabolite
DNA
Nucleoside
Thymidine
Tritium
Autoradiography
Biosynthesis
Cell division
Human
In vitro study
Kidney
Metabolism
Antimetabolites
Autoradiography
Cell Division
DNA
Human
In Vitro
Kidney
Nucleosides
Thymidine
Tritium
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TNO identifier
226946
ISSN
0014-4827
Source
Experimental Cell Research, 41 (41), 438-451
Document type
article