Print Email Facebook Twitter 2D and 3D assessment of neuropathology in rat brain after prenatal exposure to methylazoxymethanol, a model for developmental neurotoxicty Title 2D and 3D assessment of neuropathology in rat brain after prenatal exposure to methylazoxymethanol, a model for developmental neurotoxicty Author de Groot, D.M.G. Hartgring, S. van de Horst, L. Moerkens, M. Otto, M. Bos-Kuijpers, M.H.M. Kaufmann, W.S.H. Lammers, J.H.C.M. O'Callaghan, J.P. Waalkens-Berendsen, I.D.H. Pakkenberg, B. Gundersen, H.G. TNO Kwaliteit van Leven Publication year 2005 Abstract To evaluate the ability of a tiered quantitative morphological approach to reveal developmental neurotoxicity, morphometric parameters were measured in the offspring of rats treated with methylazoxymethanol (MAM) during days 13-15 of pregnancy. Treatment was aimed at inhibiting the proliferation phase of hippocampal neurons while leaving cerebellar neurons unaffected. 2D and 3D assessment of brain morphology combined with straightforward measurement of brain size, weight and volume, and the usefulness of estimation of total neuron numbers were studied. Each tier indicated major effects of MAM, from macroscopic effects in the cerebrum (first tier) to a considerable loss of neurons in the hippocampal CA1 pyramidal layer (third tier). The cerebellum and the number of cerebellar granular neurons were not changed. Along with each step of the proposed tiered approach (brain size → linear morphometry → stereology), the discriminative strength of the endpoints, and thus the probability to pinpoint the extent and location of developmental brain lesions increased. © 2005 Elsevier Inc. All rights reserved. Subject BiologyToxicology and Applied PharmacologyCerebellumDevelopmental neurotoxicityHippocampusLinear morphometryMethylazoxymethanolNeuron numbersRatStereologymethylazoxymethanolanimal experimentanimal modelanimal tissuearticlebrain developmentbrain sizebrain weightcell countcell proliferationcell structurecerebellumcontrolled studydevelopmental disorderevaluationfemalehippocampuslinear systemmalemorphometricsneuropathologyneurotoxicitynonhumanpregnancyprenatal exposureprogenypyramidal nerve cellquantitative analysisratstereologystrengththree dimensional imagingAbnormalities, Drug-InducedAnimalsAnimals, Outbred StrainsBrainCell CountFemaleImaging, Three-DimensionalInjections, IntraperitonealMaleMaternal ExposureMethylazoxymethanol AcetateOrgan SizePregnancyPrenatal Exposure Delayed EffectsRatsRats, WistarSpecific Pathogen-Free OrganismsTeratogensToxicity Tests To reference this document use: http://resolver.tudelft.nl/uuid:4d5e20d9-00fa-4043-970a-2b6b85598c05 DOI https://doi.org/10.1016/j.reprotox.2005.04.006 TNO identifier 238697 ISSN 0890-6238 Source Reproductive Toxicology, 20 (3), 417-432 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.