Title
Spatial and temporal regulation of DNA fragmentation in the aleurone of germinating barley
Author
Wang, M.
Oppedijk, B.J.
Caspers, M.P.M.
Lamers, G.E.M.
Boot, M.J.
Geerlings, D.N.G.
Bakhuizen, B.
Meijer, A.H.
van Duijn, B.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
1998
Abstract
During germination of barley grains, the appearance of DNA fragmentation started in aleurone cells near the embryo and extended to the distal end in a time-dependent manner. DNA fragmentation was demonstrated to occur only after the expression of α-amylase mRNA in the aleurone layer. In addition, cell wall degradation started in cells near the embryo on the sides facing the endosperm. Subsequently cell wall degradation extended to the lateral cell walls and to cells more to the distal end of the grain. A typical alteration of the nucleus was observed by electron microscopy and an almost complete degradation of DNA was found in the nucleus while the nuclear envelope remained intact. The results indicate that programmed cell death occurred in aleurone cells during germination. A model is proposed for the regulation of programmed cell death in aleurone cells during germination involving ABA levels and cell wall degradation.
Subject
Nutrition
Call Centre
Multimedia
Knowledge Systems
CTI
Digital Television
Speech technology
Barley aleurone
Cell walls
Hydrolytic enzymes
Programmed cell death
To reference this document use:
http://resolver.tudelft.nl/uuid:4addc861-86e1-4630-813b-7c4ebcec2509
TNO identifier
41216
ISSN
0022-0957
Source
Journal of Experimental Botany, 49 (325), 1293-1301
Document type
article