Title
Comprehensive LC-MSE lipidomic analysis using a shotgun approach and its application to biomarker detection and identification in osteoarthritis patients
Author
Castro-Perez, J.M.
Kamphorst, J.
de Groot, J.
Lafeber, F.
Goshawk, J.
Yu, K.
Shockcor, J.P.
Vreeken, R.J.
Hankemeier, T.
TNO Kwaliteit van Leven
Publication year
2010
Abstract
A fast and robust method for lipid profiling utilizing liquid chromatography coupled with mass spectrometry has been demonstrated and validated for the analysis of human plasma. This method allowed quantification and identification of lipids in human plasma using parallel alternating low energy and high energy collision spectral acquisition modes. A total of 284 lipids were identified and quantified (as relative concentrations) in both positive and negative ion electrospray ionization mode. The method was validated with five nonendogenous lipids, and the linearity (r2 better than 0.994) and the intraday and interday repeatability (relative standard deviation, 4-6% and 5-8%, respectively) were satisfactory. The developed lipid profiling method was successfully applied for the analysis of plasma from osteoarthritis (OA) patients. The multivariate statistical analysis by partial least-squares-discrimination analysis suggested an altered lipid metabolism associated with osteoarthritis and the release of arachidonic acid from phospholipids. © 2010 American Chemical Society.
Subject
Biology
Biomarker
Lipids
Mass spectrometry
Metabolite profiling
MS<sup>E</sup>
Osteoarthritis
UPLC
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http://resolver.tudelft.nl/uuid:4a6e0317-8f8a-4251-85fb-dc709c643fd7
DOI
https://doi.org/10.1021/pr901094j
TNO identifier
352046
ISSN
1535-3893
Source
Journal of Proteome Research, 9 (5), 2377-2389
Document type
article