Title
Increased matrix metalloproteinase-8 and -9 activity in patients with infarct rupture after myocardial infarction
Author
van den Borne, S.W.M.
Cleutjens, J.P.M.
Hanemaaijer, R.
Creemers, E.E.
Smits, J.F.M.
Daemen, M.J.A.P.
Blankesteijn, W.M.
TNO Kwaliteit van Leven
Publication year
2009
Abstract
Background: Infarct rupture is a usually fatal complication of myocardial infarction (MI), for which no molecular mechanism has been described in humans. Experimental evidence in mouse models suggests that the degradation of the extracellular matrix by matrix metalloproteinases (MMPs) plays an important role in infarct rupture. The present study was designed to study the role of MMP-2, MMP-8, and MMP-9 in human infarct rupture. Methods: Heart samples were obtained from patients who died from infarct rupture and control MI patients. The MMP activity was determined by zymography and quantitative immunocapture activity assay. TIMP-1 levels were measured and immunohistochemistry for MMP-2 and MMP-9 was performed. Results: The amounts of both total and active MMP-8 and MMP-9 were significantly higher in ruptured infarct tissue than in control MI tissue, but no differences in MMP-2 activity were observed. Furthermore, the number of inflammatory cells was significantly higher in the ruptured infarcts than in control infarcts. Conclusions: These data suggest that increased MMP-8 and MMP-9 activity in the infarct area, caused by a more prominent infiltration of inflammatory cells, contribute to infarct rupture in humans. © 2009 Elsevier Inc. All rights reserved.
Subject
Biology
Biomedical Research
Infarct rupture
Inflammation
Matrix metalloproteinases
MMP activity assay
MMP immunohistochemistry
Myocardial infarction
gelatinase A
gelatinase B
neutrophil collagenase
tissue inhibitor of metalloproteinase 1
adult
aged
article
clinical article
controlled study
enzyme activity
female
heart
heart disease
heart infarction
human
human tissue
immunohistochemistry
infarct rupture
inflammatory cell
male
priority journal
zymography
Aged
Aged, 80 and over
Cell Count
Female
Heart Rupture, Post-Infarction
Humans
Immunohistochemistry
Male
Matrix Metalloproteinase 2
Matrix Metalloproteinase 8
Matrix Metalloproteinase 9
Middle Aged
Myocardial Infarction
Myocarditis
To reference this document use:
http://resolver.tudelft.nl/uuid:4a4c0093-9831-47a1-929b-5f3a5de0038d
DOI
https://doi.org/10.1016/j.carpath.2007.12.012
TNO identifier
241315
ISSN
1054-8807
Source
Cardiovascular Pathology, 18 (1), 37-43
Document type
article