Print Email Facebook Twitter Increased matrix metalloproteinase-8 and -9 activity in patients with infarct rupture after myocardial infarction Title Increased matrix metalloproteinase-8 and -9 activity in patients with infarct rupture after myocardial infarction Author van den Borne, S.W.M. Cleutjens, J.P.M. Hanemaaijer, R. Creemers, E.E. Smits, J.F.M. Daemen, M.J.A.P. Blankesteijn, W.M. TNO Kwaliteit van Leven Publication year 2009 Abstract Background: Infarct rupture is a usually fatal complication of myocardial infarction (MI), for which no molecular mechanism has been described in humans. Experimental evidence in mouse models suggests that the degradation of the extracellular matrix by matrix metalloproteinases (MMPs) plays an important role in infarct rupture. The present study was designed to study the role of MMP-2, MMP-8, and MMP-9 in human infarct rupture. Methods: Heart samples were obtained from patients who died from infarct rupture and control MI patients. The MMP activity was determined by zymography and quantitative immunocapture activity assay. TIMP-1 levels were measured and immunohistochemistry for MMP-2 and MMP-9 was performed. Results: The amounts of both total and active MMP-8 and MMP-9 were significantly higher in ruptured infarct tissue than in control MI tissue, but no differences in MMP-2 activity were observed. Furthermore, the number of inflammatory cells was significantly higher in the ruptured infarcts than in control infarcts. Conclusions: These data suggest that increased MMP-8 and MMP-9 activity in the infarct area, caused by a more prominent infiltration of inflammatory cells, contribute to infarct rupture in humans. © 2009 Elsevier Inc. All rights reserved. Subject BiologyBiomedical ResearchInfarct ruptureInflammationMatrix metalloproteinasesMMP activity assayMMP immunohistochemistryMyocardial infarctiongelatinase Agelatinase Bneutrophil collagenasetissue inhibitor of metalloproteinase 1adultagedarticleclinical articlecontrolled studyenzyme activityfemaleheartheart diseaseheart infarctionhumanhuman tissueimmunohistochemistryinfarct ruptureinflammatory cellmalepriority journalzymographyAgedAged, 80 and overCell CountFemaleHeart Rupture, Post-InfarctionHumansImmunohistochemistryMaleMatrix Metalloproteinase 2Matrix Metalloproteinase 8Matrix Metalloproteinase 9Middle AgedMyocardial InfarctionMyocarditis To reference this document use: http://resolver.tudelft.nl/uuid:4a4c0093-9831-47a1-929b-5f3a5de0038d DOI https://doi.org/10.1016/j.carpath.2007.12.012 TNO identifier 241315 ISSN 1054-8807 Source Cardiovascular Pathology, 18 (1), 37-43 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.