Title
The presence of two S-layer-protein-encoding genes is conserved among species related to Lactobacillus acidophilus
Author
Boot, H.J.
Kolen, C.P.A.M.
Pot, B.
Kersters, K.
Pouwels, P.H.
Prins Maurits Laboratorium TNO
Publication year
1996
Abstract
Previously we have shown that the type strain of Lactobacillus acidophilus possesses two S-protein-encoding genes, one of which is silent, on a chromosomal segment of 6 kb. The S-protein-encoding gene in the expression site can be exchanged for the silent S-protein-encoding gene by inversion of this slp segment. In this study the presence of S-protein and corresponding S-protein-encoding genes of strains belonging to species that are closely related to L. acidophilus was determined. All strains investigated were identified by numerical comparison of highly standardized one-dimensional SDS-PAGE whole-cellular-protein patterns. Western blot and Southern blot methods were used to identify the presence of, and homology between, S-proteins and S-protein encoding genes. From these analyses we conclude that strains of L. acidophilus, L. crispatus, L. amylovorus and L. gallinarum possess an S-layer and contain two slp genes. Strains of L. helveticus possess an S-layer but have only one intact slp gene. Strains of L. gasseri, L. johnsonii and L. delbrueckii subsp. bulgaricus have neither an S-layer nor S-protein-encoding genes hybridizing with probes derived from the L. acidophilus slpA or slpB region. The presence of a highly conserved 5' region in the slp genes of strains of L. acidophilus, L. crispatus, L. amylovorus and L. gallinarum suggests that S-layer variation is a common feature for strains of these species.
Subject
Biology
S-layer variation
S-protein
SDS-PAGE identification
Surface layer
Bacterial protein
Bacterial gene
Chromosome inversion
Genetic conservation
Immunoblotting
Nonhuman
Polyacrylamide gel electrophoresis
Priority journal
Silent gene
Southern blotting
Bacterial Proteins
Blotting, Southern
Blotting, Western
Chromosome Mapping
Electrophoresis, Polyacrylamide Gel
Membrane Proteins
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Bacteria (microorganisms)
Lactobacillus
Lactobacillus acidophilus
Lactobacillus amylovorus
Lactobacillus crispatus
Lactobacillus delbrueckii subsp. bulgaricus
Lactobacillus gallinarum
Lactobacillus gasseri
Lactobacillus helveticus
Lactobacillus johnsonii
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http://resolver.tudelft.nl/uuid:491a0f5e-9984-4d0a-bdf7-78a93e9351a2
TNO identifier
233434
ISSN
1350-0872
Source
Microbiology, 142 (9), 2375-2384
Document type
article