Title
Isolation and storage of DNA for population studies
Author
Droog, S.
Lakenberg, N.
Meulenbelt, I.
de Maat, M.P.M.
Huisman, L.G.M.
Jie, A.F.H.
Slagboom, P.E.
Gaubius Laboratory TNO Preventie en Gezondheid
Publication year
1996
Abstract
For genetic population studies, human genomic DNA is commonly isolated from peripheral blood. A fast, non-invasive DNA sampling method is developed involving oral samples taken with cotton swabs. In addition various procedures were compared for isolation of DNA from different sources: whole blood or buffy-coats stored at -20°C for 5-10 years or buccal cells collected freshly with the non-invasive method. The differences in these procedures, which do not contain a phenol-extraction, are based on the use of either 1) high concentration ammonium acetate followed by DNA precipitation or 2) high concentration potassium acetate, followed by chloroform extraction and normal DNA precipitation.
Subject
Biology
dna
conference paper
human
population research
priority journal
storage
To reference this document use:
http://resolver.tudelft.nl/uuid:48a8e43a-a472-4e4e-b5d4-6312c68b5159
TNO identifier
233522
ISSN
0268-9499
Source
Fibrinolysis, 10 (SUPPL. 2), 29-30
Document type
article