Title
mRNA levels and methylation patterns of the tyrosine aminotransferase gene in aging inbred rats
Author
Instituut voor Experimentele Gerontologie TNO
Slagboom, P.E.
Leeuw, W.J.F.de
Vijg, J.
Publication year
1990
Abstract
We have examined the mRNA levels and methylation patterns of the liver-specific tyrosine aminotransferase (TAT) gene in inbred female rats aged 6, 24 and 36 months. Northern hybridization analysis of total RNA showed a 65% decrease in the steady state transcript level of TAT in the liver of 24- and 36-month old rats as compared to 6-month old animals. The TAT gene as studied by Southern hybridization analysis using the isoschizomers Hpa II and Msp I was found to be hypomethylated in the liver as compared to spleen and brain at six CpG sites within the gene. Methylation at these sites remained unchanged during aging.
Subject
DNA methylation
mRNA
Tyrosine aminotransferase
Aging
Animal
Blotting, Northern
Blotting, Southern
Gene Expression
Genes, Structural
Liver
Methylation
Rats
RNA, Messenger
Tyrosine Transaminase
Animalia
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DOI
https://doi.org/10.1016/0014-5793(90)81136-c
TNO identifier
231214
ISSN
0014-5793
Source
FEBS Letters, 269 (269), 128-130
Document type
article