Title
Unlocking Streptomyces spp. for use as sustainable industrial production platforms by morphological engineering
Author
TNO Kwaliteit van Leven
van Wezel, G.P.
Krabben, P.
Traag, B.A.
Keijser, B.J.F.
Kerste, R.
Vijgenboom, E.
Heijnen, J.J.
Kraal, B.
Publication year
2006
Abstract
Filamentous actinomycetes are commercially widely used as producers of natural products (in particular antibiotics) and of industrial enzymes. However, the mycelial lifestyle of actinomycetes, resulting in highly viscous broths and unfavorable pellet formation, has been a major bottleneck in their commercialization. Here we describe the successful morphological engineering of industrially important streptomycetes through controlled expression of the morphogene ssgA. This led to improved growth of many industrial and reference streptomycetes, with fragmentation of the mycelial clumps resulting in significantly enhanced growth rates in batch fermentations of Streptomyces coelicolor and Streptomyces lividans. Product formation was also stimulated, with a twofold increase in yield of enzyme production by S. lividans. We anticipate that the use of the presented methodology will make actinomycetes significantly more attractive as industrial and sustainable production hosts. Copyright © 2006, American Society for Microbiology. All Rights Reserved. Chemicals / CAS: Bacterial Proteins; Culture Media; ssgA protein, bacterial
Subject
Biotechnology
Antibiotics
Batch cell culture
Enzymes
Fermentation
Growth kinetics
Microbiology
Morphology
Industrial enzymes
Morphological engineering
Natural products
Pellet formation
Bacteria
Antibiotic agent
Industrial enzyme
Natural product
Antibiotics
Bacterium
Enzyme
Fermentation
Gene expression
Growth rate
Morphology
Actinobacteria
Batch fermentation
Bioengineering
Controlled study
Culture medium
Environmental sustainability
Enzyme synthesis
Fungal gene
Fungus growth
Gene expression
Morphological engineering
Mycelium
Nonhuman
SsgA gene
Streptomyces
Streptomyces coelicolor
Streptomyces lividans
Bacterial Proteins
Culture Media
Fermentation
Gene Expression Regulation, Bacterial
Genetic Engineering
Industrial Microbiology
Streptomyces
Actinobacteria (class)
Streptomyces
Streptomyces coelicolor
Streptomyces lividans
Streptomycineae
To reference this document use:
http://resolver.tudelft.nl/uuid:45467fdb-6760-471f-b9ef-35f6d9e61b6c
DOI
https://doi.org/10.1128/aem.00808-06
TNO identifier
239412
ISSN
0099-2240
Source
Applied and Environmental Microbiology, 72 (72), 5283-5288
Document type
article