Title
Identification of microorganisms grown in blood culture flasks using liquid chromatography-tandem mass spectrometry
Author
Berendsen, E.M.
Levin, E.
Braakman, R.B.H.
van der Riet-Van Oeveren, D.
Sedee, N.J.
Paauw, A.
Publication year
2017
Abstract
Aim: Bloodstream infections are a common cause of disease and a fast and accurate identification of the causative agent or agents of bloodstream infections would aid the start of adequate treatment. Materials & methods: A liquid chromatography-tandem mass spectrometry (LC-MS/MS) shotgun proteomics method was developed for the identification of bacterial species directly from blood cultures that were simulated by inoculating blood culture bottles with single or multiple Clinically relevant microorganisms. Results: Using LC-MS/MS, the single species were correctly identified in 100% of the blood cultures, whereas for polymicrobial infections, 78% of both species were correctly identified in blood cultures. Conclusion: The LC-MS/MS method allows for the identification of the causative agent of positive blood cultures. © 2017 2017 Netherlands Organization for Applied Scientific Research TNO.
Subject
bloodstream infection
identification
LC-MS/MS
Safety and Security
Defence, Safety and Security
2015 Observation, Weapon & Protection Systems
CBRN - CBRN Protection
TS - Technical Sciences
To reference this document use:
http://resolver.tudelft.nl/uuid:45139c3f-127d-4157-b54e-831a00dad8cc
DOI
https://doi.org/10.2217/fmb-2017-0050
TNO identifier
781915
ISSN
1746-0913
Source
Future Microbiology, 12 (12), 1135-1145
Document type
article