Title
Differentiation of adipose stem cells by nucleus pulposus cells: Configuration effect
Author
Lu, Z.F.
Zandieh Doulabi, B.
Wuisman, P.I.
Bank, R.A.
Helder, M.N.
TNO Kwaliteit van Leven
Publication year
2007
Abstract
Degenerative disc disease (DDD) is a major cause of chronic low back pain. For mild/intermediate DDD, regeneration by injecting adipose stem cells (ASCs) into the nucleus pulposus (NP) may be considered. The goal of this study is to investigate whether NP cells can direct ASCs towards the NP phenotype. Interactions between NP cells and ASCs were studied in transwell co-cultures, employing both monolayer and micromass configurations. Micromass culturing significantly up-regulated aggrecan and collagen type II gene expression in NP cells. In ASCs, expression of these genes and of osteopontin, collagen type I and PPAR-γ were not significantly affected. Strikingly, only when both cell types were micromass-cultured, ASCs could be chondrogenically differentiated, as shown by induction of collagen type II and aggrecan, and concomitant down-regulation of osteopontin, collagen type I and PPAR-γ. We conclude that ASCs can be directed towards the NP cell-like phenotype by soluble factor(s) secreted by NP cells. © 2007 Elsevier Inc. All rights reserved.
Subject
Biomedical Research
Adipose tissue
Chondrogenic differentiation
Co-culture
Disc regeneration
Gene expression
Mesenchymal stem cells
Nucleus pulposus
aggrecan
collagen type 1
collagen type 3
osteopontin
peroxisome proliferator activated receptor gamma
adipose tissue cell
chondrogenesis
controlled study
down regulation
gene expression
genetic analysis
monolayer culture
nucleus pulposus
phenotype
priority journal
real time polymerase chain reaction
stem cell
upregulation
Adipocytes
Cell Culture Techniques
Cell Differentiation
Cells, Cultured
Coculture Techniques
Humans
Intervertebral Disk
Stem Cells
To reference this document use:
http://resolver.tudelft.nl/uuid:44f25f32-44ad-48c4-8870-4498d725a1c1
DOI
https://doi.org/10.1016/j.bbrc.2007.06.002
TNO identifier
240135
ISSN
0006-291X
Source
Biochemical and Biophysical Research Communications, 359 (4), 991-996
Document type
article