Print Email Facebook Twitter Potency of isothiocyanates to induce luciferase reporter gene expression via the electrophile-responsive element from murine glutathione S-transferase Ya Title Potency of isothiocyanates to induce luciferase reporter gene expression via the electrophile-responsive element from murine glutathione S-transferase Ya Author Vermeulen, M. Boerboom, A.M.M.J.F. Blankvoort, B.M.G. Aarts, J.M.M.J.G. Rietjens, I.M.C.M. van Bladeren, P.J. Vaes, W.H.J. TNO Kwaliteit van Leven Publication year 2009 Abstract Isothiocyanates are electrophiles that are able to induce phase II biotransformation enzyme gene expression via an electrophile-responsive element (EpRE) in the gene regulatory region. To study the potency of different isothiocyanates to induce the expression of EpRE-regulated genes, a Hepa-1c1c7 luciferase reporter cell line was exposed to structurally different isothiocyanates. The reporter cell line, EpRE(mGST-Ya)-LUX, contains the EpRE from the regulatory region of the mouse glutathione S-transferase Ya gene. Isothiocyanates containing a methyl-sulfur side chain, e.g. sulforaphane, showed a lower EC50 (0.8-3.2 μM) and a comparable induction factor (17-22.4) compared to the structurally different isothiocyanates containing an alkyl or aromatic side chain, e.g. allyl and phenylethyl isothiocyanate (EC50 3.9-6.5 μM, induction factor 17.5-23). After 24 h of exposure, on average (±SD) 23 ± 5% of the isothiocyanate was found in the cells and 77% in the cell medium. Isothiocyanates prove to be strong inducers of electrophile-responsive element-mediated gene expression at physiological concentrations. The here described luciferase reporter cell line is a suitable assay to measure the potency of compounds to induce EpRE-regulated gene expression. © 2009 Elsevier Ltd. All rights reserved. Subject BiologyAnalytical researchAntioxidantElectrophile-responsive elementIsothiocyanateelectrophileglutathione s transferase Yaglutathione transferaseisothiocyanic acid derivativeluciferasephenyl isothiocyanatesulforaphaneunclassified druganimal cellarticlecell assaycell linechemical structureconcentration responsecontrolled studydrug potencyenzyme inductiongene expression regulationgene inductiongene locationmousenonhumanreporter geneAnimalsCell LineCell SurvivalGene Expression RegulationGenes, ReporterGlutathione TransferaseIsothiocyanatesLuciferasesMiceResponse ElementsMurinae To reference this document use: http://resolver.tudelft.nl/uuid:425a161e-cb33-486a-a38f-d453a57d532c DOI https://doi.org/10.1016/j.tiv.2009.02.005 TNO identifier 241580 ISSN 0887-2333 Source Toxicology in Vitro, 23 (4), 617-621 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.