Title
Sources of variability in the quantification of tissue optical properties by multidiameter single-fiber reflectance and fluorescence spectroscopy
Author
Brooks, S.
Hoy, C.L.
Amelink, A.
Robinson, D.J.
Nijsten, T.E.
Publication year
2015
Abstract
Recently, a multidiameter single-fiber reflectance and fluorescence spectroscopy device has been developed that enabled us to extract the autofluorescence of tissue that is corrected for the optical properties. Such a system has been incorporated in the population-based Rotterdam Study to investigate the autofluorescence of the skin. Since the device will be used by different operators over many years, it is essential that the results are comparable between users. It is, however, unclear how different methods of handling the probe might influence the outcome. Variability of blood oxygen saturation, blood volume fraction and vessel diameter, average gamma, reduced scattering coefficient at 800 nm, and integrated intrinsic fluorescence measured in three volunteers were assessed within and between eight untrained users. A variability of less than one standard deviation from the group mean was defined as an acceptable limit. Three mature volunteers were also included to assess the intrauser variability of mature skin. The variation in the measured parameters suggests that variation is dominated by tissue heterogeneity. Most users measured within one standard deviation of the group mean. Notably, corrected intrinsic fluorescence showed low intra- and interuser variability. These results strongly suggest that variability is mostly caused by tissue heterogeneity and is not user induced.
Subject
TS - Technical Sciences
Nano Technology
Industrial Innovation
High Tech Systems & Materials
Fluorescence spectroscopy
Lasers
Optical properties
Optics
Reflection
Spectroscopy
Blood oxygen saturation
Intrinsic fluorescence
Measured parameters
Reduced scattering coefficients
Sources of variability
Tissue heterogeneity
Tissue optical properties
OPT - Optics
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http://resolver.tudelft.nl/uuid:41423a8d-57d7-4972-a996-a952f3677430
TNO identifier
525592
Publisher
SPIE
ISSN
1083-3668
Source
Journal of Biomedical Optics, 20 (20)
Document type
article